Glycoconjugate Journal

, Volume 26, Issue 6, pp 663–673

UDP-Gal: N-acetylglucosamine β 1–4 galactosyltransferase expressing live attenuated parasites as vaccine for visceral leishmaniasis

  • Siddhartha Kumar Bhaumik
  • Manoj Kumar Singh
  • Subir Karmakar
  • Tripti De
Article

DOI: 10.1007/s10719-008-9212-y

Cite this article as:
Bhaumik, S.K., Singh, M.K., Karmakar, S. et al. Glycoconj J (2009) 26: 663. doi:10.1007/s10719-008-9212-y

Abstract

As compared to cutaneous leishmaniasis, vaccination against visceral leishmaniasis (VL) has received limited attention. In this study, we demonstrate for the first time that an UDP-Galactose: N-acetylglucosamine β 1–4 galactosyltransferase (GenBank Accession No. EF159943) expressing attenuated LD clonal population (A-LD) is able to confer protection against the experimental challenge with the virulent LD AG83 parasite. A-LD was also effective in established leishmania infection. The vaccinated animals showed both cell mediated (in vitro T-cell proliferation, and DTH response) and humoral responses (Th1 type). These results demonstrate the potential of the attenuated clones as an immunotherapeutic and immunoprophylactic agent against visceral leishmaniasis.

Keywords

UDP-galactose/N-acetylglucosamine β 1–4 galactosyltransferase Avirulent Leishmania donovani clonal parasite Visceral leishmaniasis Sterile protection 

Abbreviations

GalT4 UDP-galactose/N-acetylglucosamine β 1–4

Galactosyltransferase

i.c

intra-cardiac

LD

Leishmania donovani

A-LD

avirulent Leishmania donovani clone

VL

visceral leishmaniasis

s.c.

sub-cutaneous

p.i

post-infection

CMI

cell mediated immunity

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Siddhartha Kumar Bhaumik
    • 1
  • Manoj Kumar Singh
    • 1
  • Subir Karmakar
    • 1
  • Tripti De
    • 1
  1. 1.Division of Infectious Disease and ImmunologyIndian Institute of Chemical BiologyKolkataIndia

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