Glycoconjugate Journal

, Volume 23, Issue 3, pp 167–173

Altered expression and glycosylation of plasma proteins in rheumatoid arthritis

Authors

  • Sunil K. Raghav
    • Institute of Genomics & Integrative Biology
  • Bhawna Gupta
    • Institute of Genomics & Integrative Biology
  • Charu Agrawal
    • Institute of Genomics & Integrative Biology
  • Ashish Saroha
    • Institute of Genomics & Integrative Biology
  • Rakha H. Das
    • Institute of Genomics & Integrative Biology
  • Ved P. Chaturvedi
    • Department of Rheumatology, R&RArmy Hospital
  • Hasi R. Das
    • Institute of Genomics & Integrative Biology
Article

DOI: 10.1007/s10719-006-7922-6

Cite this article as:
Raghav, S.K., Gupta, B., Agrawal, C. et al. Glycoconj J (2006) 23: 167. doi:10.1007/s10719-006-7922-6

Abstract

Altered glycosylation of plasma proteins has been directly implicated in the pathogenesis of rheumatoid arthritis (RA). The present study investigated the changes in the Concanavalin-A (Con-A)-bound plasma proteins in the RA patients in comparison to that of the healthy controls. Two proteins (MW ∼32 kDa and ∼62 kDa) showed an alteration in expression while an altered monosaccharide profile (high mannose) was observed in the ∼62 kDa protein in the samples collected from RA patients. The 2-dimensional polyacrylamide gel electrophoresis analysis of the Con-A-bound plasma samples showed a large number of protein spots, a few of which were differentially expressed in the RA patients. Some unidentified proteins were detected in the RA patients which were absent in the control samples. The present study, therefore, enunciates the role of carbohydrates as well as that of the acute phase response in the disease pathogenesis.

Keywords

Rheumatoid arthritisConcanavalin AAltered glycosylationPlasma proteins2-dimensional gel electrophoresis

Copyright information

© Springer Science + Business Media, LLC 2006