Original Papers

Glycoconjugate Journal

, Volume 23, Issue 5, pp 345-354

Glycomic studies of Drosophila melanogaster embryos

  • Simon J. NorthAffiliated withDivision of Molecular Biosciences, Faculty of Life Sciences, Imperial College
  • , Kate KolesAffiliated withDepartment of Biochemistry and Biophysics, Texas A & M University
  • , Caleb HembdAffiliated withDepartment of Biochemistry and Biophysics, Texas A & M University
  • , Howard R. MorrisAffiliated withDivision of Molecular Biosciences, Faculty of Life Sciences, Imperial CollegeM-SCAN Mass Spectrometry Research and Training Centre
  • , Anne DellAffiliated withDivision of Molecular Biosciences, Faculty of Life Sciences, Imperial College
  • , Vladislav M. PaninAffiliated withDepartment of Biochemistry and Biophysics, Texas A & M University
  • , Stuart M. HaslamAffiliated withDivision of Molecular Biosciences, Faculty of Life Sciences, Imperial College Email author 

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Abstract

With the complete genome sequence of Drosophila melanogaster defined a systematic approach towards understanding the function of glycosylation has become possible. Structural assignment of the entire Drosophila glycome during specific developmental stages could provide information that would shed further light on the specific roles of different glycans during development and pinpoint the activity of certain glycosyltransferases and other glycan biosynthetic genes that otherwise might be missed through genetic analyses. In this paper the major glycoprotein N- and O-glycans of Drosophila embryos are described as part of our initial undertaking to characterize the glycome of Drosophila melanogaster. The N-glycans are dominated by high mannose and paucimannose structures. Minor amounts of mono-, bi- and tri-antennary complex glycans were observed with GlcNAc and Galβ1–4GlcNAc non-reducing end termini. O-glycans were restricted to the mucin-type core 1 Galβ1-3GalNAc sequence.

Keywords

Drosophila melanogaster Mass spectrometry Glycomics Sialic acid