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Chromosome mapping of repetitive DNAs in sergeant major fishes (Abudefdufinae, Pomacentridae): a general view on the chromosomal conservatism of the genus

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Abstract

Species of the Abudefduf genus (sergeant-majors) are widely distributed in the Indian, Pacific and Atlantic oceans, with large schools inhabiting rocky coastal regions and coral reefs. This genus consists of twenty recognized species are of generalist habit, showing typical characteristics of colonizers. Some populations maintain gene flow between large oceanic areas, a condition that may influence their cytogenetic features. A number of species have been shown to be invaders and able to hybridize with local species. However, cytogenetic data in this genus are restricted to few species. In this way, the present study includes the chromosomal investigation, using conventional (Giemsa staining, Ag-NOR and C-banding) and molecular (in situ mapping of six different repetitive DNA classes) approaches in four Abudefduf species from different oceanic regions (A. bengalensis and A. sexfasciatus from the Indo-Pacific, A. vaigiensis from the Indian and A. saxatilis from the Atlantic oceans, respectively), to investigate the evolutionary events associated with the chromosomal diversification in this group. All species share a similar karyotype (2n = 48; NF = 52), except A. sexfasciatus (2n = 48; NF = 50), which possesses a characteristic pericentric inversion in the NOR-bearing chromosomal pair. Mapping of repetitive sequences suggests a chromosomal conservatism in this genus. The high karyotypic similarity between allopatric species of Abudefduf may be related to the success of natural viable hybrids among species with recent secondary contact.

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Cooper et al. (2009) and Quenouille et al. (2004, 2011)

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Acknowledgments

This study was supported by the Toxic Substances in Livestock and Aquatic Animals Research Group, Khon Kaen University and Development and Promotion of Science and Technology talents project (DPST) and by the Brazilian agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq and Fundação de Amparo à Pesquisa do Estado de São Paulo—FAPESP.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Khon Kaen University, Muang, Khon Kaen, Thailand

    Nuntaporn Getlekha, Nuntiya Maneechot & Alongklod Tanomtong

  2. Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, São Paulo, Brazil

    Marcelo de Bello Cioffi, Cassia Fernanda Yano & Luiz Antonio Carlos Bertollo

  3. Department of Fisheries, Faculty of Applied Science and Engineering, Khon Kaen University, Nong Khai Campus, Muang, Nong Khai, Thailand

    Weerayuth Supiwong

  4. Toxic Substances in Livestock and Aquatic Animals Research Group, Khon Kaen University, Muang, Khon Kaen, 40002, Thailand

    Alongklod Tanomtong

  5. Departamento de Biologia Celular e Genética, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil

    Wagner Franco Molina

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  1. Nuntaporn Getlekha
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  2. Marcelo de Bello Cioffi
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Correspondence to Wagner Franco Molina.

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Getlekha, N., Cioffi, M.d., Yano, C.F. et al. Chromosome mapping of repetitive DNAs in sergeant major fishes (Abudefdufinae, Pomacentridae): a general view on the chromosomal conservatism of the genus. Genetica 144, 567–576 (2016). https://doi.org/10.1007/s10709-016-9925-y

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