Genetica

, Volume 140, Issue 7, pp 277–285

Cross-species amplification of microsatellites reveals incongruence in the molecular variation and taxonomic limits of the Pilosocereus aurisetus group (Cactaceae)

  • Evandro M. Moraes
  • Manolo F. Perez
  • Mariana F. Téo
  • Daniela C. Zappi
  • Nigel P. Taylor
  • Marlon C. Machado
Article

DOI: 10.1007/s10709-012-9678-1

Cite this article as:
Moraes, E.M., Perez, M.F., Téo, M.F. et al. Genetica (2012) 140: 277. doi:10.1007/s10709-012-9678-1
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Abstract

The Pilosocereus aurisetus group contains eight cactus species restricted to xeric habitats in eastern and central Brazil that have an archipelago-like distribution. In this study, 5–11 microsatellite markers previously designed for Pilosocereus machrisii were evaluated for cross-amplification and polymorphisms in ten populations from six species of the P. aurisetus group. The genotypic information was subsequently used to investigate the genetic relationships between the individuals, populations, and species analyzed. Only the Pmac101 locus failed to amplify in all of the six analyzed species, resulting in an 88 % success rate. The number of alleles per polymorphic locus ranged from 2 to 12, and the most successfully amplified loci showed at least one population with a larger number of alleles than were reported in the source species. The population relationships revealed clear genetic clustering in a neighbor-joining tree that was partially incongruent with the taxonomic limits between the P. aurisetus and P. machrisii species, a fact which parallels the problematic taxonomy of the P. aurisetus group. A Bayesian clustering analysis of the individual genotypes confirmed the observed taxonomic incongruence. These microsatellite markers provide a valuable resource for facilitating large-scale genetic studies on population structures, systematics and evolutionary history in this group.

Keywords

Taxonomic uncertainties Xeric vegetation Rocky outcrop habitats Closely related species 

Supplementary material

10709_2012_9678_MOESM1_ESM.doc (302 kb)
Supplementary material 1 (DOC 302 kb)
10709_2012_9678_MOESM2_ESM.doc (38 kb)
Supplementary material 2 (DOC 37 kb)

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Evandro M. Moraes
    • 1
  • Manolo F. Perez
    • 1
  • Mariana F. Téo
    • 1
  • Daniela C. Zappi
    • 2
    • 5
  • Nigel P. Taylor
    • 3
  • Marlon C. Machado
    • 4
  1. 1.Departamento de BiologiaUniversidade Federal de São CarlosSorocabaBrazil
  2. 2.Royal Botanic Gardens, KewRichmondUK
  3. 3.National Parks BoardGardens by the Bay and Singapore Botanic GardensSingaporeSingapore
  4. 4.Departamento de Ciências BiológicasUniversidade Estadual de Feira de SantanaFeira de SantanaBrazil
  5. 5.Gardens by the BaySingaporeSingapore