Genetica

, Volume 139, Issue 11, pp 1465–1475

Adaptive evolution of Xa21 homologs in Gramineae

Authors

  • Shengjun Tan
    • State Key Laboratory of Pharmaceutical Biotechnology, School of Life SciencesNanjing University
  • Dan Wang
    • State Key Laboratory of Pharmaceutical Biotechnology, School of Life SciencesNanjing University
  • Jing Ding
    • State Key Laboratory of Pharmaceutical Biotechnology, School of Life SciencesNanjing University
  • Dacheng Tian
    • State Key Laboratory of Pharmaceutical Biotechnology, School of Life SciencesNanjing University
    • State Key Laboratory of Pharmaceutical Biotechnology, School of Life SciencesNanjing University
    • Department of BiologyNanjing University
    • State Key Laboratory of Pharmaceutical Biotechnology, School of Life SciencesNanjing University
    • Department of BiologyNanjing University
Article

DOI: 10.1007/s10709-012-9645-x

Cite this article as:
Tan, S., Wang, D., Ding, J. et al. Genetica (2011) 139: 1465. doi:10.1007/s10709-012-9645-x

Abstract

The XA21 protein has broad spectrum resistance against Xanthomonas oryzae pv. oryzae. Although Xa21-mediated immunity is well characterized, little is known about the origin and evolutionary history of this gene in grasses. Therefore, we analyzed all Xa21 gene homologs in eight whole-genome sequenced rice lines, as well as in four gramineous genomes, rice, Brachypodium, sorghum and maize; using Arabidopsis Xa21 homologs as outgroups, 17, 7, 7 and 3 Xa21 homologs were detected in these four grasses, respectively. Synteny and phylogenetic analysis showed that frequent gene translocation, duplication and/or loss, have occurred at Xa21 homologous loci, suggesting that they have undergone or are undergoing rapid generation of copy number variations. Within the rice species, the high level of nucleotide diversity between Xa21-like orthologs showed a strong association with the presence/absence haplotypes, suggesting that the genetic structure of rice lines plays an important role in the variations between these Xa21-like orthologs. Strongly positive selection was detected in the core region of the leucine-rich repeat domains of the Xa21 subclade among the rice lines, indicating that the rapid gene diversification of Xa21 homologs may be a strategy for a given species to adapt to the changing spectrum of species-specific pathogens.

Keywords

Xa21 Adaptive evolution Syntenic block Positive selection

Supplementary material

10709_2012_9645_MOESM1_ESM.doc (2.2 mb)
Supplementary material 1 (DOC 2229 kb)

Copyright information

© Springer Science+Business Media B.V. 2012