Genetica

, 139:961

A single major QTL controls expression of larval Cry1F resistance trait in Ostrinia nubilalis (Lepidoptera: Crambidae) and is independent of midgut receptor genes

  • Brad S. Coates
  • Douglas V. Sumerford
  • Miriam D. Lopez
  • Haichuan Wang
  • Lisa M. Fraser
  • Jeremy A. Kroemer
  • Terrence Spencer
  • Kyung S. Kim
  • Craig A. Abel
  • Richard L. Hellmich
  • Blair D. Siegfried
Article

DOI: 10.1007/s10709-011-9590-0

Cite this article as:
Coates, B.S., Sumerford, D.V., Lopez, M.D. et al. Genetica (2011) 139: 961. doi:10.1007/s10709-011-9590-0

Abstract

The European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae), is an introduced crop pest in North America that causes major damage to corn and reduces yield of food, feed, and biofuel materials. The Cry1F toxin from Bacillus thuringiensis (Bt) expressed in transgenic hybrid corn is highly toxic to O. nubilalis larvae and effective in minimizing feeding damage. A laboratory colony of O. nubilalis was selected for high levels of Cry1F resistance (>12,000-fold compared to susceptible larvae) and is capable of survival on transgenic hybrid corn. Genetic linkage maps with segregating AFLP markers show that the Cry1F resistance trait is controlled by a single quantitative trait locus (QTL) on linkage group 12. The map position of single nucleotide polymorphism (SNP) markers indicated that midgut Bt toxin-receptor genes, alkaline phosphatase, aminopeptidase N, and cadherin, are not linked with the Cry1F QTL. Evidence suggests that genes within this genome interval may give rise to a novel Bt toxin resistance trait for Lepidoptera that appears independent of known receptor-based mechanisms of resistance.

Keywords

Quantitative trait locus (QTL)Amplified fragment length polymorphism (AFLP)Single nucleotide polymorphism (SNP)

Supplementary material

10709_2011_9590_MOESM1_ESM.doc (34 kb)
Supplementary material 1 (DOC 34 kb)
10709_2011_9590_MOESM2_ESM.txt (59 kb)
Supplementary material 2 (TXT 58 kb)
10709_2011_9590_MOESM3_ESM.txt (58 kb)
Supplementary material 3 (TXT 57 kb)
10709_2011_9590_MOESM4_ESM.doc (34 kb)
Supplementary material 4 (DOC 34 kb)

Copyright information

© Springer 2011

Authors and Affiliations

  • Brad S. Coates
    • 1
    • 5
  • Douglas V. Sumerford
    • 1
    • 2
  • Miriam D. Lopez
    • 1
    • 2
  • Haichuan Wang
    • 3
  • Lisa M. Fraser
    • 2
  • Jeremy A. Kroemer
    • 1
  • Terrence Spencer
    • 3
  • Kyung S. Kim
    • 4
  • Craig A. Abel
    • 1
    • 2
  • Richard L. Hellmich
    • 1
    • 2
  • Blair D. Siegfried
    • 3
  1. 1.USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics LaboratoryIowa State UniversityAmesUSA
  2. 2.Department of EntomologyIowa State UniversityAmesUSA
  3. 3.Department of EntomologyUniversity of NebraskaLincolnUSA
  4. 4.Department of Agricultural BiotechnologySeoul National UniversitySeoulRepublic of Korea
  5. 5.USDA-ARS, Corn Insects & Crop Genetics Research, 113 Genetics LabIowa State UniversityAmesUSA