Article

Genetica

, Volume 138, Issue 7, pp 709-716

Construction of a BAC library and a physical map of a major QTL for CBB resistance of common bean (Phaseolus vulgaris L.)

  • S. Y. LiuAffiliated withAgriculture Agri-Food Canada, Greenhouse and Processing Crops Research CenterCrop and Soil Environmental Science, Virginia Polytechnic Institute and State University
  • , K. YuAffiliated withAgriculture Agri-Food Canada, Greenhouse and Processing Crops Research Center Email author 
  • , M. HuffnerAffiliated withAgriculture Agri-Food Canada, Greenhouse and Processing Crops Research Center
  • , S. J. ParkAffiliated withAgriculture Agri-Food Canada, Greenhouse and Processing Crops Research Center
  • , M. BanikAffiliated withAgriculture Agri-Food Canada, Greenhouse and Processing Crops Research CenterCereal Research Center, Agriculture and Agri-Food Canada
  • , K. P. PaulsAffiliated withDepartment of Plant Agriculture, Crop Science Building, University of Guelph
  • , W. CrosbyAffiliated withDepartment of Biology, University of Windsor

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Abstract

A major quantitative trait loci (QTL) conditioning common bacterial blight (CBB) resistance in common bean (Phaseolus vulgaris L.) lines HR45 and HR67 was derived from XAN159, a resistant line obtained from an interspecific cross between common bean lines and the tepary bean (P. acutifolius L.) line PI319443. This source of CBB resistance is widely used in bean breeding. Several other CBB resistance QTL have been identified but none of them have been physically mapped. Four molecular markers tightly linked to this QTL have been identified suitable for marker assisted selection and physical mapping of the resistance gene. A bacterial artificial chromosome (BAC) library was constructed from high molecular weight DNA of HR45 and is composed of 33,024 clones. The size of individual BAC clone inserts ranges from 30 kb to 280 kb with an average size of 107 kb. The library is estimated to represent approximately sixfold genome coverage. The BAC library was screened as BAC pools using four PCR-based molecular markers. Two to seven BAC clones were identified by each marker. Two clones were found to have both markers PV-tttc001 and STS183. One preliminary contig was assembled based on DNA finger printing of those positive BAC clones. The minimum tiling path of the contig contains 6 BAC clones spanning an estimated size of 750 kb covering the QTL region.

Keywords

Common bacterial blight Physical mapping Bacterial artificial chromosome library Common bean Contig