Genetica

, 135:67

Characterization of new hAT transposable elements in 12 Drosophila genomes

  • Mauro de Freitas Ortiz
  • Elgion Lucio Silva Loreto
Article

DOI: 10.1007/s10709-008-9259-5

Cite this article as:
de Freitas Ortiz, M. & Loreto, E.L.S. Genetica (2009) 135: 67. doi:10.1007/s10709-008-9259-5

Abstract

In silico searches for sequences homologous to hAT elements in 12 Drosophila genomes have allowed us to identify 37 new hAT elements (8 in D. ananassae, 11 in D. mojavensis, 2 in D. sechellia, 1 in D. simulans, 2 in D. virilis, 3 in D. yakuba, 3 in D. persimilis, 1 in D. grimshawi, 5 in D. willistoni and 1 in D. pseudobscura). The size of these elements varies from 2,359 to 4,962 bp and the terminal inverted repeats (TIRs) show lengths ranging from 10 to 24 bp. Several elements show intact transposase ORFs, suggesting that they are active. Conserved amino acid motifs were identified that correspond to those important for transposase activity. These elements are highly variable and phylogenetic analysis showed that they can be clustered into four different families. Incongruencies were observed between the phylogenies of the transposable elements and those of their hosts, suggesting that horizontal transfer may have occurred between some of the species.

Keywords

hAT transposonsTransposable elementTransposase motifsHorizontal transfer

Supplementary material

10709_2008_9259_MOESM1_ESM.pdf (95 kb)
Supplementary figures (PDF 217 kb)

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Mauro de Freitas Ortiz
    • 1
  • Elgion Lucio Silva Loreto
    • 2
  1. 1.Curso de Ciências BiológicasUniversidade Federal de Santa MariaSanta MariaBrazil
  2. 2.Departamento de Biologia, CCNEUniversidade Federal de Santa MariaSanta MariaBrazil