Genetica

, Volume 124, Issue 1, pp 61–69

A new image of plantain diversity assessed by SSR, AFLP and MSAP markers

  • J. L. Noyer
  • S. Causse
  • K. Tomekpe
  • A. Bouet
  • F. C. Baurens
Article

DOI: 10.1007/s10709-004-7319-z

Cite this article as:
Noyer, J.L., Causse, S., Tomekpe, K. et al. Genetica (2005) 124: 61. doi:10.1007/s10709-004-7319-z

Abstract

Using both SSR and AFLP markers, the genetic diversity of 30 plantains constituting a representative sample of the phenotypic diversity was assessed. The results confirmed a very narrow genetic base of this cultivar group. SSR and AFLP data support the hypothesis that these cultivars may have arisen from vegetative multiplication of a single seed. MSAP were used to survey cytosine methylation status at CCGG sites in order to obtain an alternative source of diversity data. A higher degree of polymorphism was revealed allowing the classification of the samples into three clusters. No correlation was observed between the phenotypic classification and methylation diversity. Implications for breeding programs are discussed.

Keywords

DNA methylationgenetic diversityMusa sp.polyploidyvegetative multiplication

Abbreviations

AFLP

Amplified Fragment Length Polymorphism

MSAP

Methylation Sensitive Amplification Polymorphism

SSR

Simple Sequence Repeat

RFLP

Restriction Fragment Length Polymorphism

RAPD

Random Amplified Polymorphic DNA

CARBAP

Centre Africain de Recherches sur Bananiers et Plantains

CTAB

Cetyltrimethylammoniumbromide

Copyright information

© Springer 2005

Authors and Affiliations

  • J. L. Noyer
    • 1
  • S. Causse
    • 2
  • K. Tomekpe
    • 3
  • A. Bouet
    • 1
  • F. C. Baurens
    • 2
  1. 1.CIRADUMR 1096 PIAFrance
  2. 2.CIRADUMR 1098 BEPC, TA 40/03Montpellier cedex 5France
  3. 3.Centre Africain de Recherches sur Bananiers et Plantains (CARBAP)DoualaCameroun