Genetica

, Volume 122, Issue 3, pp 311–324

Molecular and genetic organization of Drosophila melanogasterpolytene chromosomes: evidence for two types of interband regions

Authors

  • Sergei Demakov
    • Institute of Cytology and Genetics
  • Andrei Gortchakov
    • Institute of Cytology and Genetics
  • Yuri Schwartz
    • Department of ZoologyUniversity of Geneva
  • Valery Semeshin
    • Institute of Cytology and Genetics
  • Sonsoles Campuzano
    • Centro de Biología Molecular Severo OchoaUniversidad Autonoma de Madrid
  • Juan Modolell
    • Centro de Biología Molecular Severo OchoaUniversidad Autonoma de Madrid
  • Igor Zhimulev
    • Institute of Cytology and Genetics
Article

DOI: 10.1007/s10709-004-2839-0

Cite this article as:
Demakov, S., Gortchakov, A., Schwartz, Y. et al. Genetica (2004) 122: 311. doi:10.1007/s10709-004-2839-0

Abstract

The 3A and 60E regions of Drosophila melanogaster polytene chromosomes containing inserted copies of the P{lArB} transposon have been subjected to an electron microscopic (EM) analysis. We show that both inserts led to formation of new bands within the interband regions 3A4/A6 and 60E8-9/E10. This allowed us to clone DNA of these interbands. Their sequences, as well as those of DNA from other four interbands described earlier, have been analyzed. We have found that, with the exception of 60E8-9/E10 interband, all other five regions under study corresponded to 5' or 3' ends of genes. We have further obtained the evidence for 60E8-9/E10 interband to harbor the 'housekeeping' RpL19 gene, which is transcribed in many tissues, including salivary glands. Based upon the genetic heterogeneity of the interbands observed a revised model of polytene chromosome organization is discussed.

Drosophila electron microscopy interband polytene chromosome P transposon

Copyright information

© Kluwer Academic Publishers 2004