Nutrient Cycling in Agroecosystems

, Volume 88, Issue 3, pp 329–339

Quantification of symbiotic nitrogen fixation by Elaeagnus angustifolia L. on salt-affected irrigated croplands using two 15N isotopic methods


    • Center for Development Research (ZEF)
  • J. P. A. Lamers
    • Center for Development Research (ZEF)
  • C. Martius
    • Inter-American Institute for Global Change Research (IAI)
  • A. Khamzina
    • Center for Development Research (ZEF)
  • N. Ibragimov
  • P. L. G. Vlek
    • Center for Development Research (ZEF)
Original Article

DOI: 10.1007/s10705-010-9357-5

Cite this article as:
Djumaeva, D., Lamers, J.P.A., Martius, C. et al. Nutr Cycl Agroecosyst (2010) 88: 329. doi:10.1007/s10705-010-9357-5


Afforestation with fast growing N-fixing trees is an option for ecological restoration of highly-salinized irrigated croplands, but information about the N-fixing capability of trees on saline soils is sparse. The 15N-enrichment technique (15NET) and the A value (AV) method were used to quantify in lysimeters the proportion of atmospheric N2 (%Ndfa) fixed by Elaeagnus angustifolia L., with a reference to non-N-fixing Gleditsia triacanthos L. and Ulmus pumila L. Twenty kg N ha−1 of 5 atom %15N excess ammonium nitrate (35% N) was applied to 1-year-old trees in 2007 and 2-year-olds in 2008. Since this rate was insufficient for the older reference trees, 60 kg N ha−1 was applied in 2008. With 15NET, the %Ndfa of E. angustifolia in 2007 was 79% when referenced against U. pumila and 68% against G. triacanthos. With the AV method, the %Ndfa of 2-year-old E. angustifolia was 80 and 68% when referenced against U. pumila and G. triacanthos, respectively. Over 2 years, E. angustifolia fixed 17 kg N ha−1 when related to U. pumila and 14 kg N ha−1 with G. triacanthos (assumed density: 5,000 trees ha−1). N-fixing E. angustifolia has the potential to be self-sufficient in N when planted in the strongly saline soils.


Atom %15N excessBNFLysimetersLabeling methodSoil fertilityUzbekistan

Copyright information

© Springer Science+Business Media B.V. 2010