Methane Oxidation in Pig and Cattle Slurry Storages, and Effects of Surface Crust Moisture and Methane Availability
Received: 04 April 2005 Accepted: 27 September 2005 DOI:
10.1007/s10705-005-3822-6 Cite this article as: Petersen, S.O. & Ambus, P. Nutr Cycl Agroecosyst (2006) 74: 1. doi:10.1007/s10705-005-3822-6 Abstract
Storages with liquid manure (slurry) may develop a surface crust of particulate organic matter, or an artificial crust can be established. Slurry storages are net sources of atmospheric methane (CH
4), but a potential for bacterial oxidation of CH 4 in surface crusts was recently suggested in a study of experimental storages. The present study was conducted to investigate methanotrophic activity under practical storage conditions. Surface crusts from slurry storages at two pig farms and four dairy farms were sampled in late autumn. Mixed samples (0–4 cm depth) were used to determine changes in CH 4, O 2 and CO 2 during incubation, while intact subsamples were used to characterize CH 4 oxidation as a function of CH 4 availability and moisture content. Methane oxidation was observed in all materials except for an expanded clay product (Leca) sampled from a pig slurry storage. Despite significant variation between replicate subsamples, there was a significant increase in methanotrophic activity when CH 4 concentrations increased from 500 to 50,000 ppmv. Maximum fluxes ranged from −1 to −4.5 g CH 4 m −2 d −1. Surface crust samples were partly dried and then re-wetted in four steps to the original moisture content, each time followed by determination of CH 4 fluxes. Only one surface crust material showed a relationship between CH 4 fluxes and moisture content that would implicate gas diffusivity in the regulation of CH 4 oxidation. The occurrence of inducible CH 4 oxidation activity in slurry storage surface crusts indicates that there is a potential for stimulating the process by manipulation of gas phase composition above the stored slurry. Keywords Respiratory quotient Methane oxidation Slurry storage Storage cover References Amaral, J.A., Archambault, C., Richards, S.R., Knowles, R. 1995 Denitrification associated with Groups I and II methanotrophs in a gradient enrichment system FEMS Microbiol. Ecol. 18 289 298 Google Scholar Ambus, P., Petersen, S.O. 2005 Oxidation of 13C-labeled methane in surface crusts Isotop. Environm. Health Studies 41 125 133 Google Scholar Anthonisen, A.C., Loehr, R.C., Prakasam, T.B.S., Srinath, E.G. 1976 Inhibition of nitrification by ammonia and nitrous acid J. Water Poll. Cont. Fed. 48 835 852 Google Scholar Ball, B.C., Dobbie, K.E., Parker, J.P., Smith, K.A. 1997a The influence of gas transport and porosity on methane oxidation in soils J. Geophys. Res. 102 23301 23308 Google Scholar Ball, B.C., Smith, K.A., Klemedtsson, L., Brumme, R., Sitaula, B.K., Hansen, S., Prieme, A., MacDonald, J., Horgan, G.W. 1997b The influence of soil gas transport properties on methane oxidation in a selection of northern European soils J. Geophys. Res. 102 23309 23317 Google Scholar Bodelier, P.L.E., Laanbroek, H.J. 2004 Nitrogen as a regulatory factor of methane oxidation in soils and sediments FEMS Microbiol. Ecol. 47 265 277 Google Scholar Chanton, J.P., Whiting, G.J. 1995 Trace gas exchange in freshwater and coastal marine environments: ebullition and transport by plants Matson, P.A. Harriss, R.C. eds. Biogenic Trace Gases: Measuring Emissions from Soil and Water Blackwell Science Ltd. Oxford, UK 98 125 Google Scholar
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