Article

Fish Physiology and Biochemistry

, Volume 39, Issue 4, pp 895-905

Partial characterization and ontogenetic development of pancreatic digestive enzymes in Japanese eel Anguilla japonica larvae

  • Koji MurashitaAffiliated withNational Research Institute of Aquaculture, Fisheries Research Agency Email author 
  • , Hirofumi FuruitaAffiliated withNational Research Institute of Aquaculture, Fisheries Research Agency
  • , Hiroyuki MatsunariAffiliated withNational Research Institute of Aquaculture, Fisheries Research Agency
  • , Takeshi YamamotoAffiliated withNational Research Institute of Aquaculture, Fisheries Research Agency
  • , Masahiko AwajiAffiliated withNational Research Institute of Aquaculture, Fisheries Research Agency
  • , Kazuharu NomuraAffiliated withNational Research Institute of Aquaculture, Fisheries Research Agency
  • , Jiro NagaoAffiliated withNational Research Institute of Aquaculture, Fisheries Research Agency
  • , Hideki TanakaAffiliated withNational Research Institute of Aquaculture, Fisheries Research Agency

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Abstract

The pancreatic digestive enzymes, trypsin, chymotrypsin, lipase and amylase were partially characterized, and changes in their activities were examined during the initial ontogeny of Japanese eel Anguilla japonica larvae from 5 to 34 days post-hatching (dph). The pH optima of the eel larval enzymes were narrower than those other fish species; trypsin activity was highest at pH 9, chymotrypsin and amylase activities were highest at pH 7 and 8, and lipase activity was highest at pH 8 and 9. In an analysis of thermal profiles, the larval pancreatic enzymes had a high optimal temperature and high thermal stability, which are typical of fish from the tropics. At 12 and 13 dph, lipase activity and gene expression levels of trypsin (-a and -b), lipase and amylase decreased markedly, suggesting a marked change in larval metabolism at that time. These data could be useful in the development of artificial larval diets in Japanese eel.

Keywords

Japanese eel Larvae Pancreatic digestive enzymes Activity Gene expression