Fish Physiology and Biochemistry

, Volume 38, Issue 6, pp 1553–1563

Channel catfish, Ictalurus punctatus (Rafinesque), tetraspanin membrane protein family: identification, characterization and phylogenetic analysis of tetraspanin 3 and tetraspanin 7 (CD231) transcripts

Article

DOI: 10.1007/s10695-012-9645-x

Cite this article as:
Yeh, H. & Klesius, P.H. Fish Physiol Biochem (2012) 38: 1553. doi:10.1007/s10695-012-9645-x

Abstract

Tetraspanins, a large cell surface protein superfamily characterized by having four transmembrane domains, play many critical roles in physiological and pathological processes. In this study, we report the identification, characterization and phylogenetic analysis of the channel catfish tetraspanin 3 and tetraspanin 7 (CD231) transcripts. The full-length nucleotide sequences of tetraspanin 3 and tetraspanin 7 cDNA have 1,453 and 1,842 base pairs, respectively. Analysis of the nucleotide sequences reveals that each has one open reading frame (ORF). The ORF of tetraspanin 3 appears to encode 241 amino acids with calculated molecular mass of 26.8 kDa, while the ORF of tetraspanin 7 potentially encodes 251 amino acids with calculated molecular mass of 27.9 kDa. By comparison with the human counterparts, the channel catfish tetraspanin 3 and tetraspanin 7 peptides have four transmembrane domains, three intracellular domains and two (small and large) extracellular domains. In addition, several characteristic features critical for structure and functions in mammalian tetraspanins are also conserved in channel catfish tetraspanin 3 and tetraspanin 7. The transcripts were detected by RT-PCR in restrictive organs. These results with those from our previous studies on other channel catfish tetraspanins provide important information for further investigating the roles of various tetraspanins in channel catfish infection with microorganisms.

Keywords

Channel catfishIctalurus punctatusTetraspanin 3TSPAN3Tetraspanin 7TSPAN7CD231

Copyright information

© Springer Science+Business Media B.V. (outside the USA)  2012

Authors and Affiliations

  1. 1.Aquatic Animal Health Research Unit, Agricultural Research ServiceUnited States Department of AgricultureAuburnUSA
  2. 2.Poultry Microbiological Safety Research UnitRichard B. Russell Research Center, Agricultural Research Service, United States Department of AgricultureAthensUSA