Fish Physiology and Biochemistry

, 31:303

Hematology and stress physiology of juvenile diploid and triploid shortnose sturgeon (Acipenser brevirostrum)

Authors

  • M. M. Beyea
    • Department of BiologyUniversity of New Brunswick
    • Department of BiologyUniversity of New Brunswick
  • J. D. Kieffer
    • Department of BiologyUniversity of New Brunswick
Article

DOI: 10.1007/s10695-005-1552-y

Cite this article as:
Beyea, M.M., Benfey, T.J. & Kieffer, J.D. Fish Physiol Biochem (2005) 31: 303. doi:10.1007/s10695-005-1552-y
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Abstract

This study examined diploid and triploid shortnose sturgeon hematology and stress physiology through the investigation of various characteristics and components of whole blood and blood plasma. Erythrocytic cellular and nuclear length and width were significantly larger in triploids than in diploids. Hematocrit was depressed in triploid sturgeon in comparison to diploids, but total blood hemoglobin content and mean erythrocytic hemoglobin concentration (MEHC) did not differ between ploidies. The mean erythrocytic hemoglobin (MEH) was elevated in proportion to the increase in erythrocyte size. Taken together, these data suggest that triploids and diploids likely have similar oxygen carrying and aerobic capacities. In response to an acute stressor of 15 min chasing, plasma cortisol and glucose levels did not differ between ploidies. Cortisol levels were significantly depressed at 2 h post-stress with an increase back to 0 h post-stress levels at 6 h into recovery, whereas glucose levels did not change during the recovery period. There was a significant interaction between ploidy and time post-stress for blood hemoglobin concentrations, with diploids demonstrating elevated hemoglobin content at 6 h post-stress. Plasma osmolality, chloride ion concentrations and lactate levels were elevated in triploids. Overall, it does not appear that sturgeon have developed an elaborate stress response and the triploid stress response appears to be slightly reduced in comparison to diploids.

Keywords

cortisolforced exercisehematologylactateosmolalitytriploidy

Abbreviations

ELISA

enzyme linked immunosorbent assay

GLM

general linear model

Hb

total hemoglobin concentration

Hct

hematocrit

MEH

mean erythrocytic hemoglobin

MEHC

mean erythrocytic hemoglobin concentration

MEV

mean erythrocytic volume

PIT

passively integrated transponder

RBC

red blood cell

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© Springer 2006