Evolutionary Ecology

, Volume 24, Issue 3, pp 541–553

Experimental alteration of DNA methylation affects the phenotypic plasticity of ecologically relevant traits in Arabidopsis thaliana

  • Oliver Bossdorf
  • Davide Arcuri
  • Christina L. Richards
  • Massimo Pigliucci
Original Paper

DOI: 10.1007/s10682-010-9372-7

Cite this article as:
Bossdorf, O., Arcuri, D., Richards, C.L. et al. Evol Ecol (2010) 24: 541. doi:10.1007/s10682-010-9372-7


Heritable phenotypic variation in plants can be caused not only by underlying genetic differences, but also by variation in epigenetic modifications such as DNA methylation. However, we still know very little about how relevant such epigenetic variation is to the ecology and evolution of natural populations. We conducted a greenhouse experiment in which we treated a set of natural genotypes of Arabidopsis thaliana with the demethylating agent 5-azacytidine and examined the consequences of this treatment for plant traits and their phenotypic plasticity. Experimental demethylation strongly reduced the growth and fitness of plants and delayed their flowering, but the degree of this response varied significantly among genotypes. Differences in genotypes’ responses to demethylation were only weakly related to their genetic relatedness, which is consistent with the idea that natural epigenetic variation is independent of genetic variation. Demethylation also altered patterns of phenotypic plasticity, as well as the amount of phenotypic variation observed among plant individuals and genotype means. We have demonstrated that epigenetic variation can have a dramatic impact on ecologically important plant traits and their variability, as well as on the fitness of plants and their ecological interactions. Epigenetic variation may thus be an overlooked factor in the evolutionary ecology of plant populations.


Arabidopsis thaliana5-azacytidineDNA methylationEpigeneticsNatural variationPhenotypic plasticity

Supplementary material

10682_2010_9372_MOESM1_ESM.pdf (20 kb)
Supplementary material 1 (PDF 19 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Oliver Bossdorf
    • 1
  • Davide Arcuri
    • 2
  • Christina L. Richards
    • 3
  • Massimo Pigliucci
    • 4
  1. 1.Institute of Plant SciencesUniversity of BernBernSwitzerland
  2. 2.Dipartimento di Biotecnologie (Bio.M.A.A.)Università Mediterranea di Reggio CalabriaReggio CalabriaItaly
  3. 3.Department of Integrative BiologyUniversity of South FloridaTampaUSA
  4. 4.Department of PhilosophyCity University of New York-Lehman CollegeBronxUSA