Abstract
The varieties with erect panicles used in rice breeding have led to a significant increase in rice yield. But the molecular mechanism of erect panicle remains elusive. Oryza glumaepatula, with the erect panicle phenotype, is an excellent gene pool to improve Asian cultivated rice O. sativa. In this study, a backcross introgression line was derived with Dianjingyou 1, a japonica cultivar, as the recurrent parent and an accession of O. glumaepatula as the donor parent. Using BC5F2 population, a novel gene responsible for erect panicle, EP4, was mapped within a 1.5 cM region flanked by SSR markers RM253 and RM19623 on the short arm of chromosome 6, at genetic distances of 0.4 and 1.1 cM, respectively. These results are useful for marker-assisted selection of erect panicles and for further cloning of the EP4 gene, which will contribute to the dissection of the molecular mechanism underlying erect panicles in rice.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chen WF, Xu ZJ, Zhang LB, Zhang WZ, Ma DR (2007) Theories and practices of breeding japonica rice for super high yield. Sci Agric Sin 40:869–874
Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19:1349
Gao SJ, Chen WF, Zhang BL (1999) Studies of erect panicle in rice. J Jilin Agric Sci 24:12–15
Huang X, Qian Q, Liu Z, Sun H, He S, Luo D, Xia G, Chu C, Li J, Fu X (2009) Natural variation at the DEP1 locus enhances grain yield in rice. Nat Genet 41:494–497
Khush GS (1997) Origin, dispersal, cultivation and variation of rice. Plant Mol Biol 35:25–34
Li F, Liu W, Tang J, Chen J, Tong H, Hu B, Li C, Fang J, Chen M, Chu C (2010) Rice DENSE AND ERECT PANICLE 2 is essential for determining panicle outgrowth and elongation. Cell Res 20:838–849
Lincoln S, Daly M and Lander ES (1992) Constructing genetic maps with MAPMAKER/EXP3.0. 3rd edn. Technical report, Whitehead Institute
McCouch SR, Teytelman L, Xu Y, Lobos KB, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L (2002) Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.) (supplement). DNA Res 9:257–279
Piao R, Jiang W, Ham TH, Choi MS, Qiao Y, Chu SH, Park JH, Woo MO, Jin Z, An G, Lee J, Koh HJ (2009) Map-based cloning of the ERECT PANICLE 3 gene in rice. Theor Appl Genet 119:1497–1506
Qiao Y, Piao R, Shi J, Lee SI, Jiang W, Kim BK, Lee J, Han L, Ma W, Koh HJ (2011) Fine mapping and candidate gene analysis of dense and erect panicle 3, DEP3, which confers high grain yield in rice (Oryza sativa L.). Theor Appl Genet 122:1439–1449
Ren F, Lu BR, Li S, Huang J, Zhu Y (2003) A comparative study of genetic relationships among the AA-genome Oryza species using RAPD and SSR markers. Theor Appl Genet 108:113–120
Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, Swapan D, Ishiyama K, Saito T, Kobayashi M, Khush GS, Kitano H, Matsuoka M (2002) Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416:701–702
Spielmeyer W, Ellis MH, Chandler PM (2002) Semidwarf (sd-1), “green revolution” rice, contains a defective gibberellin 20-oxidase gene. Proc Natl Acad Sci 99:9043–9048
Tanksley SD, McCouch SR (1997) Seed banks and molecular maps: unlocking genetic potential from the wild. Science 277:1063–1066
Wang Y, Li J (2008) Molecular basis of plant architecture. Annu Rev Plant Biol 59:253–279
Wang J, Nakazaki T, Chen S, Chen W, Saito H, Tsukiyama T, Okumoto Y, Xu Z, Tanisaka T (2009) Identification and characterization of the erect-pose panicle gene EP conferring high grain yield in rice (Oryza sativa L.). Theor Appl Genet 119:85–91
Xu ZJ, Chen WF, Zhang LB, Yang SR (2005) Design principles and parameters of rice ideal panicle type. Chin Sci Bull 50:2253–2256
Yan CJ, Zhou JH, Yan S, Chen F, Yeboah M, Tang SZ, Liang GH, Gu MH (2007) Identification and characterization of a major QTL responsible for erect panicle trait in japonica rice (Oryza sativa L.). Theor Appl Genet 115:1093–1100
Yi X, Zhang Z, Zeng S, Tian C, Peng J, Li M, Lu Y, Meng Q, Gu M, Yan C (2011) Introgression of qPE9-1 allele, conferring the panicle erectness, leads to the decrease of grain yield per plant in japonica rice (Oryza sativa L.). J Genet Genomics 38:217–223
Zhang WZ, Xu ZJ, Zhang LB, Chen WF, Qiu FL, Shao GJ, Hua ZT (2002) Analysis on evolution for the erect panicle type varieties of rice. J Shenyang Agric Univ 33:161–166
Zhou J, Xu Y, Xu P, Deng X, Hu F, Li J, Ren G, Tao D (2008) Introgression and mapping of erect panicle gene from Oryza glaberrma into Oryza Sativa. Rice Genet Newsl 24:18–21
Zhou Y, Zhu J, Li Z, Yi C, Liu J, Zhang H, Tang S, Gu M, Liang G (2009) Deletion in a quantitative trait gene qPE9-1 associated with panicle erectness improves plant architecture during rice domestication. Genetics 183:315–324
Zhu K, Tang D, Yan C, Chi Z, Yu H, Chen J, Liang J, Gu M, Cheng Z (2010) Erect panicle 2 encodes a novel protein that regulates panicle erectness in indica rice. Genetics 184:343–350
Acknowledgments
This research is supported by Grants from The National Natural Science Foundation of China (31160275, 31201196, U1036605), Yunnan Provincial Sciences and Technology Department (2011FB118, 2010CI035, 2013FA056), and Food Crops Research Institute, Yunnan academy of agricultural sciences (2013LZS001).
Author information
Authors and Affiliations
Corresponding author
Additional information
Yu Zhang, Jiawu Zhou and Ying Yang have contributed equally to this work.
Rights and permissions
About this article
Cite this article
Zhang, Y., Zhou, J., Yang, Y. et al. A novel gene responsible for erect panicle from Oryza glumaepatula . Euphytica 205, 739–745 (2015). https://doi.org/10.1007/s10681-015-1390-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10681-015-1390-7