Euphytica

, Volume 187, Issue 3, pp 359–368

Evidence for second division restitution as the basis for 2n + n maternal chromosome transmission in a sugarcane cross

  • S. R. Hermann
  • K. S. Aitken
  • P. A. Jackson
  • A. W. George
  • N. Piperidis
  • X. Wei
  • A. Kilian
  • F. Detering
Article

DOI: 10.1007/s10681-012-0698-9

Cite this article as:
Hermann, S.R., Aitken, K.S., Jackson, P.A. et al. Euphytica (2012) 187: 359. doi:10.1007/s10681-012-0698-9

Abstract

Chromosome doubling in megaspores of sugarcane was first reported over 70 years ago and was seen primarily in interspecific crosses between Saccharum officinarum and Saccharum spontaneum. Since then, there has been robust debate on the mechanism(s) responsible for this doubling with hypotheses ranging from second division restitution (SDR), post-meiotic restitution to fusion of the two innermost cells of the megaspore tetrad. In the present study, we sought to answer this question using molecular marker data from a cross which demonstrated 2n + n chromosome transmission between an S. officinarum clone (IJ76-514; 2n transmission) and a commercial hybrid (Q165Open image in new window ; n transmission). Molecular marker data from four other bi-parental crosses were used to form a “base-line” for expected segregation ratios (n + n transmission). Comparison of segregation ratios showed that 2n transmission from the S. officinarum clone could be identified by the increased segregation ratios, a characteristic of both SDR and tetrad cell fusion. Simulation was then undertaken based on current understandings of sugarcane meiosis, to determine if the observed segregation ratios matched those simulated for SDR/tetrad cell fusion. Analysis of the simulation confirmed that most and perhaps all progeny appear to have been formed from a mechanism akin to SDR/tetrad cell fusion. The genetic consequences of this are discussed.

Keywords

Sugarcane Second division restitution Post-meiotic restitution Megaspore 

Supplementary material

10681_2012_698_MOESM1_ESM.pdf (24 kb)
Supplementary material 1 (PDF 23 kb)

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • S. R. Hermann
    • 1
  • K. S. Aitken
    • 2
  • P. A. Jackson
    • 3
  • A. W. George
    • 4
  • N. Piperidis
    • 5
  • X. Wei
    • 5
  • A. Kilian
    • 6
  • F. Detering
    • 6
  1. 1.BSES LimitedIndooroopillyAustralia
  2. 2.Queensland Bioscience PrecinctCSIRO Plant IndustrySt LuciaAustralia
  3. 3.Davies LaboratoryCSIRO Plant IndustryAitkenvaleAustralia
  4. 4.Ecosciences PrecinctCSIRO Mathematics, Statistics and InformaticsBrisbaneAustralia
  5. 5.BSES LimitedCanberraAustralia
  6. 6.Diversity Arrays Technology Pty LtdYarralumlaAustralia

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