Euphytica

, Volume 181, Issue 2, pp 267–275

Molecular S-genotyping and determination of S-RNase-based incompatibility groups in loquat [Eriobotrya japonica (Thunb.) Lindl.]

  • Laura Carrera
  • Javier Sanzol
  • Esteve Soler
  • María Herrero
  • Jose I. Hormaza
Article

DOI: 10.1007/s10681-011-0426-x

Cite this article as:
Carrera, L., Sanzol, J., Soler, E. et al. Euphytica (2011) 181: 267. doi:10.1007/s10681-011-0426-x
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Abstract

Loquat (Eriobotrya japonica Lindl.; Pyrinae, Rosaceae) is an evergreen fruit tree which is acquiring increasing importance as an alternative crop in warm-temperate and subtropical regions. Recent pollination studies suggest that crop intensification has resulted in a reduction of fruit productivity in different locations. Loquat exhibits gametophytic self-incompatibility (GSI) and, consequently, fruit set strongly depends on cross-pollination between intercompatible cultivars. S-genotyping and incompatibility group assignment is therefore essential for adequate loquat orchard design. This study reports the molecular characterization of the S-RNase allele composition of 31 loquat cultivars grown in Spain. PCR with primers developed from loquat S-RNase sequences was used in combination with high-performance capillary polyacrilamide electrophoresis for the fine-evaluation of length polymorphisms at the S-RNase gene. This approach allowed to uncover a new S-RNase allele (S5), whose sequence is reported and to characterize the first six S-RNase-based incompatibility groups (I–VI) in loquat, resulting from the combination of five S-alleles (S1S5). The large number of cultivars that could be assigned to only six groups suggests a narrow genetic base for this crop in Spain and stresses the need for careful determination of the S-genotype of cultivars in an attempt to overcome production problems associated with inadequate pollination.

Keywords

Eriobotrya japonica Gametophytic self-incompatibility Loquat S-RNase S-genotype 

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Laura Carrera
    • 1
  • Javier Sanzol
    • 2
  • Esteve Soler
    • 3
  • María Herrero
    • 1
  • Jose I. Hormaza
    • 4
  1. 1.Departamento de PomologíaEstación Experimental de Aula Dei - CSICZaragozaSpain
  2. 2.Unidad de FruticulturaCentro de Investigación y Tecnología Agroalimentaria de Aragón (CITA)ZaragozaSpain
  3. 3.Cooperativa de Callosa d’en SarriàAlicanteSpain
  4. 4.Instituto de Hortofruticultura Subtropical y Mediterránea ‘La Mayora’ (IHSM-UMA-CSIC), Estación Experimental La MayoraConsejo Superior de Investigaciones CientíficasAlgarrobo-CostaSpain

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