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Identification of quantitative trait loci for Cd and Zn concentrations of brown rice grown in Cd-polluted soils

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Abstract

Low grain cadmium (Cd) and high grain zinc (Zn) rice cultivars have become the current rice breeding objectives. However, the genetic control of Cd and Zn concentrations in brown rice remains poorly understood, especially when grown in Cd-contaminated soil. In this study, quantitative trait loci (QTLs) associated with grain Cd and Zn concentrations and Cd/Zn ratio were identified using a doubled haploid population derived from a cross between japonica JX17 and indica ZYQ8 rice cultivars. Three and two QTLs were detected for Cd and Zn concentration in brown rice, respectively. Two QTLs associated with grain Cd/Zn ratio on chromosomes 3 and 6 were initially mapped. These QTLs accounted for 10.83–41.66% of the total variance of the three traits measured. Only one common locus on chromosome 6 was found for Cd concentration and Cd/Zn ratio. The lack of co-location of the QTLs for Cd and Zn concentrations in this mapping population suggests different genetic mechanisms. In summary, our results provide insight into the genetic basis of rice grain Cd and Zn accumulation; the isolated QTLs may be useful for marker-assisted selection and identification of genes associated with Cd and Zn accumulation in rice.

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References

  • Arao T, Ae N (2003) Genotypic variation in cadmium levels of rice grain. Soil Sci Plant Nutr 49:473–479

    CAS  Google Scholar 

  • Avendano B S (2000) Tagging high zinc content in the grain, and zinc deficiency tolerance genes in rice (Oryza sativa L.) using simple sequence repeats (SSR). MS thesis, Laguna College, Los Baños

  • Berg MG, Gardner EH (1978) Methods of soil analysis used in the soil testing laboratory at Oregon State University. Oregon State University. Agricultural Experiment Station, Corvallis. Special Report. 321, 44

  • Chen Z, Wang Z, He J, Zhong W, Wang J, Yang J, Zhang H (2009) Mapping of QTLs of Cd2+ content in brown rice under Cd2+ stress in rice. Hereditas 31(11):1135–1140

    PubMed  CAS  Google Scholar 

  • Cheng WD, Zhang GP, Yao HG, Wu W, Xu M (2006) Genotypic and environmental variation in cadmium, chromium, arsenic, nickel and lead concentrations in rice grains. J Zhejiang Univ Sci 7:565–571

    Article  CAS  Google Scholar 

  • Garcia-Oliveira AL, Tan L, Fu Y, Sun C (2009) Genetic identification of quantitative trait loci for contents of mineral nutrients in rice grain. J Integr Plant Biol 51(1):84–92

    Article  PubMed  CAS  Google Scholar 

  • Grant CA, Clarke JM, Duguid S, Chaney RL (2008) Selection and breeding of plant cultivars to minimize cadmium accumulation. Sci Total Environ 390:301–310

    Article  PubMed  CAS  Google Scholar 

  • Inaba T, Kobayashi E, Suwazono Y, Uetani M, Oishi M, Nakagawa H, Nogawa K (2005) Estimation of cumulative cadmium intake causing itai-itai disease. Toxicol Lett 159(2):192–201

    Article  PubMed  CAS  Google Scholar 

  • Ishikawa S, Ae N, Yano M (2005) Chromosomal regions with quantitative trait loci controlling cadmium concentration in brown rice (Oryza sativa). New Phytol 168(20):345–350

    Article  PubMed  CAS  Google Scholar 

  • Ishikawa S, Abe T, Kuramata M, Yamaguchi M, Ando T, Yamamoto T, Yano M (2010) A major quantitative trait locus for increasing cadmium-specific concentration in rice grain is located on the short arm of chromosome 7. J Exp Bot 61(3):923–934

    Article  PubMed  CAS  Google Scholar 

  • Kashiwagi T, Shindoh K, Hirotsu N, Ishimaru K (2009) Evidence for separate translocation pathways in determining cadmium accumulation in grain and aerial plant parts in rice. BMC Plant Biol 9:8

    Article  PubMed  Google Scholar 

  • Liu J, Li K, Xu J, Liang J, Lu X, Yang J, Zhu Q (2003a) Interaction of Cd and five mineral nutrients for uptake and accumulation in different rice cultivars and genotypes. Field Crop Res 83:271–281

    Article  Google Scholar 

  • Liu J, Liang J, Li K, Zhang Z, Yu B, Lu X, Yang J, Zhu Q (2003b) Correlations between cadmium and mineral nutrients in absorption and accumulation in various genotypes of rice under cadmium stress. Chemosphere 52:1467–1473

    Article  PubMed  CAS  Google Scholar 

  • Liu JG, Zhu QS, Zhang ZJ, Xu JK, Yang JC, Wong MH (2005) Variations in cadmium accumulation among rice cultivars and types and the selection of cultivars for reducing cadmium in the diet. J Sci Food Agric 85:147–153

    Article  CAS  Google Scholar 

  • Lu C, Shen L, Tan Z, Xu Y, He P, Chen Y, Zhu L (1996) Comparative mapping of QTLs for agronomic traits of rice across environments using a doubled haploid population. Theor Appl Genet 93:1211–1217

    Article  CAS  Google Scholar 

  • Lu K, Li L, Zheng X, Zhang Z, Mou T, Hu Z (2008) Quantitative trait loci controlling Cu, Ca, Zn, Mn and Fe content in rice grains. J Genet 87(3):305–310

    Article  PubMed  Google Scholar 

  • McCouch SR, Cho YG, Yano M, Paul E, Blinstrub M (1997) Report on QTL nomenclature. Rice Genet Newsl 14:11–13

    Google Scholar 

  • Nriagu JO, Pacyna JM (1988) Quantitative assessment of worldwide contamination of air, water and soils by trace metals. Nature 333:134–139

    Article  PubMed  CAS  Google Scholar 

  • Pedersen B, Eggum BO (1983) The influence of milling on the nutritive value of flour from cereal grains.4. Rice. Qual Plant Foods Hum Nutr 33:267–278

    Article  CAS  Google Scholar 

  • Shi J, Li L, Pan G (2009) Variation of grain Cd and Zn concentrations of 110 hybrid rice cultivars grown in a low-Cd paddy soil. J Environ Sci 21:168–172

    Article  CAS  Google Scholar 

  • Simmons RW, Pongsakul P, Chaney RL, Saiyasitpanich D, Klinphoklap S, Nobuntou W (2003) The relative exclusion of zinc and iron from rice grain in relation to rice grain cadmium as compared to soybean: implications for human health. Plant Soil 257:163–170

    Article  CAS  Google Scholar 

  • Stangoulis JCR, Huynh BL, Welch RM, Choi EY, Graham RD (2007) Quantitative trait loci for phytate in rice grain and their relationship with grain micronutrient content. Euphytica 154:289–294

    Article  Google Scholar 

  • Tezuka K, Miyadate H, Katou K, Kodama I, Matsumoto S, Kawamoto T, Masaki S, Satoh H, Yamaguchi M, Sakurai K, Takahashi H, Satoh-Nagasawa N, Watanabe A, Fujimura T, Akagi H (2009) A single recessive gene controls cadmium translocation in the cadmium hyperaccumulating rice cultivar Cho-Ko-Koku. Theor Appl Genet 120:1175–1182

    Article  PubMed  Google Scholar 

  • Ueno D, Kono I, Yokosho K, Ando T, Yano M, Ma JF (2009a) A major quantitative trait locus controlling cadmium translocation in rice (Oryza sativa). New Phytol 182:644–653

    Article  PubMed  CAS  Google Scholar 

  • Ueno D, Koyama E, Kono I, Ando T, Yano M, Ma JF (2009b) Identification of a novel major quantitative trait locus controlling distribution of Cd between roots and shoots in rice. Plant Cell Physiol 50:2223–2233

    Article  PubMed  CAS  Google Scholar 

  • Wang DL, Zhu J, Li ZK, Paterson AH (1999) Mapping QTLs with epistatic effects and QTLx environment interactions by mixed linear model approaches. Theor Appl Genet 99:1255–1264

    Article  Google Scholar 

  • Williams CH, David DJ (1973) The effect of superphosphate on the cadmium content of soils and plants. Aust J Soil Res 11:43–56

    Article  CAS  Google Scholar 

  • Wong SC, Li XD, Zhang G, Qi SH, Min YS (2002) Heavy metals in agricultural soil of the Pearl River Delta, South China. Environ Pollut 119:33–44

    Article  PubMed  CAS  Google Scholar 

  • Xu YB, Shen LS, McCouch SR, Zhu LH (1998) Extension of the rice DH population genetic map with microsatellite markers. Chin Sci Bull 42:149–152

    Google Scholar 

  • Xue D, Chen M, Zhang G (2009) Mapping of QTLs associated with cadmium tolerance and accumulation during seedling stage in rice (Oryza sativa L.). Euphytica 165:587–596

    Article  CAS  Google Scholar 

  • Yu H, Wang J, Fang W, Yuan J, Yang Z (2006) Cadmium accumulation in different rice cultivars and screening for pollution-safe cultivars of rice. Sci Total Environ 370:302–309

    Article  PubMed  CAS  Google Scholar 

  • Zeng FR, Mao Y, Cheng WD, Wu FB, Zhang GP (2008) Genotypic and environmental variation in chromium, cadmium and lead concentrations in rice. Environ Pollut 153:309–314

    Article  PubMed  CAS  Google Scholar 

  • Zhu LH, Chen Y, Xu YB, Xu JC, Cai HW, Ling ZZ (1993) Construction of a molecular map of rice and gene mapping using a double haploid population of a cross between Indica and Japonica varieties. Rice Genet Newsl 10:132–134

    Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (30800676; 30871513; 30921140408), and Zhejiang Natural Science Foundation (Y3090114).

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Authors and Affiliations

  1. College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, 310036, China

    Xiaoqin Zhang, Huizhong Wang, Qian Qian & Dawei Xue

  2. Agronomy Department, Zhejiang University, Hangzhou, 310029, China

    Xiaoqin Zhang & Guoping Zhang

  3. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China

    Longbiao Guo, Dali Zeng, Guojun Dong & Qian Qian

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  1. Xiaoqin Zhang
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  2. Guoping Zhang
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  3. Longbiao Guo
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  4. Huizhong Wang
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  5. Dali Zeng
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  6. Guojun Dong
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  7. Qian Qian
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  8. Dawei Xue
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Correspondence to Qian Qian or Dawei Xue.

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Zhang, X., Zhang, G., Guo, L. et al. Identification of quantitative trait loci for Cd and Zn concentrations of brown rice grown in Cd-polluted soils. Euphytica 180, 173–179 (2011). https://doi.org/10.1007/s10681-011-0346-9

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  • DOI: https://doi.org/10.1007/s10681-011-0346-9

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