Euphytica

, 171:301

Inheritance of beta-carotene-associated flesh color in cucumber (Cucumis sativus L.) fruit

  • H. E. Cuevas
  • H. Song
  • J. E. Staub
  • P. W. Simon
Review

DOI: 10.1007/s10681-009-0017-2

Cite this article as:
Cuevas, H.E., Song, H., Staub, J.E. et al. Euphytica (2010) 171: 301. doi:10.1007/s10681-009-0017-2
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Abstract

The nutritional value of cucumber (Cucumis sativus L.) can be improved by the introgression of β-carotene (i.e., provitamin A and/or orange flesh) genes from “Xishuangbanna gourd” (XIS; Cucumis sativus var. xishuangbannanesis Qi et Yuan) into US pickling cucumber. However, the genetics of β-carotene content has not been clearly defined in this US market type. Thus, three previous populations derived from a US pickling cucumber (‘Addis’) × XIS mating were evaluated for β-carotene content, from which the high β-carotene inbred line (S4), ‘EOM 402-10’, was developed. A cross was then made between the US pickling cucumber inbred line ‘Gy7’ [gynoecious, no β-carotene, white flesh; P1] and ‘EOM 402-10’ [monoecious, possessing β-carotene, orange flesh; P2] to determine the inheritance of β-carotene in fruit mesocarp and endocarp tissue. Parents and derived cross-progenies (F1, F2, BC1P1, and BC1P2) were evaluated for β-carotene content in a greenhouse in Madison, Wisconsin. While F1 and BC1P1 progeny produced mature fruits possessing white, light-green, and green (0.01–0.02 μg g−1 β-carotene) mesocarp, the F2 and BC1P2 progeny mesocarp segregated in various hues of white, green, yellow (0.01–0.34 μg g−1 β-carotene), and orange (1.90–2.72 μg g−1 β-carotene). Mesocarp and endocarp F2 segregation adequately fit a 15:1 [low-β-carotene (0.01–0.34 μg g−1): high-β-carotene (1.90–2.72 μg g−1)] and 3:1 (low-β-carotene: high-β-carotene) ratio, respectively. Likewise, segregation of carotene concentration in mesocarp and endocarp tissues in BC1P2 progeny adequately fit a 3:1 (low-β-carotene: high-β-carotene) and 1:1 (low-β-carotene: high-β-carotene) ratio, respectively. Progeny segregations indicate that two recessive genes control the β-carotene content in the mesocarp, while one recessive gene controls β-carotene content in the endocarp. Single marker analysis of F2 progeny using the carotenoid biosynthesis gene Phytoene synthase determined that there was no association between this gene and the observed β-carotene variation in either fruit mesocarp or endocarp.

Keywords

Carotenoid Beta-carotene Exotic germplasm Vitamin A Nutraceutical 

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • H. E. Cuevas
    • 1
    • 2
  • H. Song
    • 1
    • 3
  • J. E. Staub
    • 4
    • 5
  • P. W. Simon
    • 4
  1. 1.Plant Breeding and Plant Genetics Program, Department of HorticultureUniversity of WisconsinMadisonUSA
  2. 2.Plant Genome Mapping LaboratoryCenter for Applied Genetic TechnologiesAthensUSA
  3. 3.Nanjing Agricultural UniversityNanjingPeople’s Republic of China
  4. 4.U. S. Department of Agriculture, Agricultural Research Service, Vegetable Crops Unit, Plant Breeding and Plant Genetics Program, Department of HorticultureUniversity of WisconsinMadisonUSA
  5. 5.USDA, ARS Forage and Range Research LaboratoryLoganUSA

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