Euphytica

, Volume 165, Issue 1, pp 79–88

Mapping and validation of QTLs for resistance to an Indian isolate of Ascochyta blight pathogen in chickpea

Authors

  • Pratibha Kottapalli
    • International Crops Research Institute for the Semi-Arid Tropics
    • Department of BiotechnologyIndira Gandhi Agricultural University
    • International Crops Research Institute for the Semi-Arid Tropics
  • Sanjay K. Katiyar
    • Department of BiotechnologyIndira Gandhi Agricultural University
  • Jonathan H. Crouch
    • International Crops Research Institute for the Semi-Arid Tropics
  • Hutokshi K. Buhariwalla
    • International Crops Research Institute for the Semi-Arid Tropics
  • Suresh Pande
    • International Crops Research Institute for the Semi-Arid Tropics
  • Kishore K. Gali
    • International Crops Research Institute for the Semi-Arid Tropics
Article

DOI: 10.1007/s10681-008-9762-x

Cite this article as:
Kottapalli, P., Gaur, P.M., Katiyar, S.K. et al. Euphytica (2009) 165: 79. doi:10.1007/s10681-008-9762-x

Abstract

Ascochyta blight (AB) caused by Ascochyta rabiei, is globally the most important foliar disease that limits the productivity of chickpea (Cicer arietinum L.). An intraspecific linkage map of cultivated chickpea was constructed using an F2 population derived from a cross between an AB susceptible parent ICC 4991 (Pb 7) and an AB resistant parent ICCV 04516. The resultant map consisted of 82 simple sequence repeat (SSR) markers and 2 expressed sequence tag (EST) markers covering 10 linkage groups, spanning a distance of 724.4 cM with an average marker density of 1 marker per 8.6 cM. Three quantitative trait loci (QTLs) were identified that contributed to resistance to an Indian isolate of AB, based on the seedling and adult plant reaction. QTL1 was mapped to LG3 linked to marker TR58 and explained 18.6% of the phenotypic variance (R2) for AB resistance at the adult plant stage. QTL2 and QTL3 were both mapped to LG4 close to four SSR markers and accounted for 7.7% and 9.3%, respectively, of the total phenotypic variance for AB resistance at seedling stage. The SSR markers which flanked the AB QTLs were validated in a half-sib population derived from the same resistant parent ICCV 04516. Markers TA146 and TR20, linked to QTL2 were shown to be significantly associated with AB resistance at the seedling stage in this half-sib population. The markers linked to these QTLs can be utilized in marker-assisted breeding for AB resistance in chickpea.

Keywords

Ascochyta blightChickpeaCicer arietinumGenetic linkage mapQTL mappingSSR markers

Copyright information

© Springer Science+Business Media B.V. 2008