, Volume 159, Issue 1-2, pp 259-273
Date: 01 Jul 2007

Molecular and SNP characterization of two genome specific transcription factor genes GhMyb8 and GhMyb10 in cotton species

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Abstract

Two R2R3-Myb cDNAs (GhMyb8 and GhMyb10) and their corresponding genes were isolated and characterized from allotetraploid cotton (Gossypium hirsutum L. cv. DES119) fiber cells. Both GhMyb8 and GhMyb10 exhibit some conserved features shared in subgroup 4 of plant R2R3-MYB proteins, including the GIDxxH motif and a zinc-finger domain. Both genomic origin and single nucleotide polymorphism (SNP) analyses reveal that GhMyb8 and 10 are alloallelic genes in the allotetraploid cotton (AD genome). GhMyb10 is derived from the A2 subgenome, whereas GhMyb8 is from the D5 subgenome. Possible chromosomal locations of these two genes were explored by SNP marker based deletion analyses. The results showed that the average rate of SNP per nucleotide among the selected genotypes in the two gene fragments was 3.75% (∼one SNP per 27 nucleotide), and 0.55% and 5.14% in coding regions and 3′-UTR (3′ untranslated regions), respectively. Northern blot analysis showed that GhMyb8 and GhMyb10 are expressed in all examined tissues, including leaves, flowers, roots, and fibers from different developmental stages; however, the transcript level of GhMyb8/10 is more abundant in flowers and roots. The ectopic expression of GhMyb10 in transgenic tobacco plants showed the abnormal cell shapes in leaf trichomes, suggesting that GhMyb8 and GhMyb10 might play a role in the process of trichome cell differentiation. The exact chromosomal location of the two myb genes couldn’t be determined using the SNP deletion method due to the incomplete coverage of cytogenetic stocks.

The nucleotide sequences of GhMyb8 and GhMyb10 have been submitted to GenBank and assigned accession numbers EF421795 and EF421796, respectively.