Euphytica

, Volume 159, Issue 1, pp 259–273

Molecular and SNP characterization of two genome specific transcription factor genes GhMyb8 and GhMyb10 in cotton species

  • Chuan-Yu Hsu
  • Chuanfu An
  • Sukumar Saha
  • Din-Pow Ma
  • Johnie N. Jenkins
  • Brian Scheffler
  • David M. Stelly
Article

DOI: 10.1007/s10681-007-9485-4

Cite this article as:
Hsu, CY., An, C., Saha, S. et al. Euphytica (2008) 159: 259. doi:10.1007/s10681-007-9485-4

Abstract

Two R2R3-Myb cDNAs (GhMyb8 and GhMyb10) and their corresponding genes were isolated and characterized from allotetraploid cotton (Gossypiumhirsutum L. cv. DES119) fiber cells. Both GhMyb8 and GhMyb10 exhibit some conserved features shared in subgroup 4 of plant R2R3-MYB proteins, including the GIDxxH motif and a zinc-finger domain. Both genomic origin and single nucleotide polymorphism (SNP) analyses reveal that GhMyb8 and 10 are alloallelic genes in the allotetraploid cotton (AD genome). GhMyb10 is derived from the A2 subgenome, whereas GhMyb8 is from the D5 subgenome. Possible chromosomal locations of these two genes were explored by SNP marker based deletion analyses. The results showed that the average rate of SNP per nucleotide among the selected genotypes in the two gene fragments was 3.75% (∼one SNP per 27 nucleotide), and 0.55% and 5.14% in coding regions and 3′-UTR (3′ untranslated regions), respectively. Northern blot analysis showed that GhMyb8 and GhMyb10 are expressed in all examined tissues, including leaves, flowers, roots, and fibers from different developmental stages; however, the transcript level of GhMyb8/10 is more abundant in flowers and roots. The ectopic expression of GhMyb10 in transgenic tobacco plants showed the abnormal cell shapes in leaf trichomes, suggesting that GhMyb8 and GhMyb10 might play a role in the process of trichome cell differentiation. The exact chromosomal location of the two myb genes couldn’t be determined using the SNP deletion method due to the incomplete coverage of cytogenetic stocks.

Keywords

Gossypium hirsutumR2R3-MybTransgenic tobacco plantsTrichome

Supplementary material

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Chuan-Yu Hsu
    • 1
  • Chuanfu An
    • 2
  • Sukumar Saha
    • 3
  • Din-Pow Ma
    • 1
  • Johnie N. Jenkins
    • 3
  • Brian Scheffler
    • 4
    • 5
  • David M. Stelly
    • 6
  1. 1.Department of Biochemistry and Molecular BiologyMississippi State UniversityMississippi StateUSA
  2. 2.Department of Plant and Soil SciencesMississippi State UniversityMississippi StateUSA
  3. 3.USDA-ARS Crop Science Research LaboratoryMississippi StateUSA
  4. 4.USDA-ARS, USDA-CGRUStonevilleUSA
  5. 5.Department of Plant SciencesUniversity of CaliforniaDavisUSA
  6. 6.Department of Soil and Crop SciencesTexas A&M UniversityCollege StationUSA