Article

Euphytica

, Volume 141, Issue 1, pp 181-190

First online:

Role of the genes Md-ACO1 and Md-ACS1 in ethylene production and shelf life of apple (Malus domestica Borkh)

  • Fabrizio CostaAffiliated withDipartimento di Colture Arboree, University of Bologna Email author 
  • , Sara StellaAffiliated withDipartimento di Colture Arboree, University of Bologna
  • , W. Eric Van de WegAffiliated withPlant Research International
  • , Walter GuerraAffiliated withCentro per la Sperimentazione Agraria e Forestale Laimburg
  • , Michela CecchinelAffiliated withCentro per la Sperimentazione Agraria e Forestale Laimburg
  • , Joseph DallaviaAffiliated withCentro per la Sperimentazione Agraria e Forestale Laimburg
  • , Bernie KollerAffiliated withInstitute of Plant Science, Swiss Federal Institute of Technology
  • , Silviero SansaviniAffiliated withDipartimento di Colture Arboree, University of Bologna

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Shelf life determines the economic life time of mature apples, which can be either freshly harvested or stored. Good shelf life is highly associated with a slow decrease of fruit firmness at room temperature. Apple is a climacteric fruit, in which loss of firmness seems to be physiologically related to ethylene. Ethylene’s biosynthetic pathway is controlled by two large gene families coding for 1-aminocyclopropane-1-carboxylate synthase (ACS) and 1-aminocyclopropane-1-carboxylate oxydase (ACO).

In this study, one ACS and one ACO gene were examined for their effect on ethylene production and shelf life in apple using gene specific molecular marker, and have also been positioned on a molecular marker linkage map. The ACO marker was developed in this research and mapped on linkage group (LG) 10 of the crosses Prima × Fiesta and Fuji × Mondial Gala, within the 5% border of a previously identified fruit firmness QTL [Theor Appl Genet 100 (2000) 1074]. We denoted this locus as Md-ACO1. In addition, we mapped the previously developed Md-ACS1 marker [Theor Appl Genet 101 (2000) 742] on LG15.

Studies on the cross Fuji × Braeburn revealed that Md-ACS1 and Md-ACO1 independently affect the internal ethylene concentration (IEC) as well as shelf life of apple, Md-ACS1 having the strongest effect. Descendants homozygous for Md-ACS1-2 and Md-ACO1-1 showed to have the lowest ethylene production as well as superior shelf-life. These two genes are candidates to be included in marker assisted breeding.

Key words

ACO ACS ethylene production fruit softening marker assisted breeding