Environmental Monitoring and Assessment

, Volume 131, Issue 1, pp 129–134

IMS Method Performance Analyses for Giardia in Water Under Differing Conditions


DOI: 10.1007/s10661-006-9462-8

Cite this article as:
Hsu, BM. & Huang, C. Environ Monit Assess (2007) 131: 129. doi:10.1007/s10661-006-9462-8


Immunomagnetic separation (IMS) has been specified as a standard method for the measurement of Giardia under USEPA Method 1623. In this study, IMS was evaluated on the basis of recovery efficiencies for Giardia cysts under various IMS operation conditions. Significant change in recovery was observed by altering the debris ratio of water samples. Notably, cyst recovery efficiencies utilizing IMS dropped with increased turbidity, and results for varying dosages of magnetic beads and cysts indicate that 1 / 100 immunomagnetic beads is sufficient to conjugate large numbers of cysts. Changing vessel volume and replacing the sample buffer had no significant effect on cyst recovery efficiencies.


GiardiaImmunomagnetic separation (IMS)Method 1623

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.Department of Earth and Environmental SciencesNational Chung Cheng UniversityMin-Hsiung, ChiayiRepublic of China
  2. 2.Institute of Environmental EngineeringNational Chiao Tung UniversityHsinchuRepublic of China