Environmental Monitoring and Assessment

, Volume 104, Issue 1, pp 155–162

Using the Flow Cytometry to Quantify the Giardia Cysts and Cryptosporidium Oocysts in Water Samples

  • Bing-Mu Hsu
  • Nan-Min Wu
  • Hung-Der Jang
  • Feng-Cheng Shih
  • Min-Tao Wan
  • Chien-Min Kung
Article

DOI: 10.1007/s10661-005-1608-6

Cite this article as:
Hsu, B., Wu, N., Jang, H. et al. Environ Monit Assess (2005) 104: 155. doi:10.1007/s10661-005-1608-6

Abstract

The flow cytometry (FC) has been used to detect Giardia cysts and Cryptosporidium oocysts quantitatively and instantaneously in this study. The experimental results showed that FC is potential to become a more precise method for the detection of Giardia and Cryptosporidium in water. This study also evaluated the staining efficiencies for three commercial antibodies. After staining Cryptosporidium oocysts with direct immunofluorescent antibodies in water samples, two populations were detected in the scatter-plots (FL1 versus SSC) of the FC. The Cryptosporidium oocysts and Giardia cysts are significantly separated from other particles while stained with direct immunofluorescent antibodies produced by Meridian Diagnostics and WaterborneTM Inc.

Keywords

Cryptosporidiumflow cytometryGiardiaprotozoan parasites

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Bing-Mu Hsu
    • 1
    • 2
  • Nan-Min Wu
    • 2
  • Hung-Der Jang
    • 3
  • Feng-Cheng Shih
    • 2
  • Min-Tao Wan
    • 2
  • Chien-Min Kung
    • 4
  1. 1.Department of Bioenvironmental EngineeringChung Yuan Christian UniversityChung-LiTaiwan
  2. 2.Department of Environmental Engineering and HealthYuanpei University of Science and TechnologyHsinchuTaiwan
  3. 3.Department of Food ScienceYuanpei University of Science and TechnologyHsinchuTaiwan
  4. 4.Department of Medical TechnologyYuanpei University of Science and TechnologyHsinchuTaiwan