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Characterization of Pseudoperonospora cubensis isolates from Europe and Asia using ISSR and SRAP molecular markers

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Abstract

Downy mildew caused by Pseudoperonospora cubensis is a major disease of cucurbits worldwide. New genotypes of the pathogen have recently appeared in the USA, EU and Israel causing breakdown of genetic resistance, expansion of host range, and the appearance of a new A2 mating type. Seventy-eight P. cubensis isolates were collected during 1996–2011 from cucurbits fields in different regions of Turkey, Israel and the Czech Republic and genetic diversity was analysed using highly polymorphic ISSR and SRAP molecular markers. The data acquired showed remarkable genetic diversity within and among the isolates. While isolates from Turkey and Czech Republic exhibited uniform genetic background, the isolates from Israel were clearly distinguished from the others. The results may indicate on migration and/or frequent sexual reproduction of the pathogen in Israel. Moreover the selected markers can be suggested for monitoring genetic diversity within P. cubensis isolates in further studies.

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Acknowledgments

The research of IP and OB, FM, YC, FC was supported by Republic of Turkey Ministry of Food, Agriculture and Livestock Projects (TAGEM-BS-10/10-11/02-09). MK and AL were supported by Palacký University funds (IGA PrF-2014-001), by MSM 6198959215 (Ministry of Education, Youth and Sports of the Czech Republic) and QH 71229 (Czech Ministry of Agriculture).

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Authors and Affiliations

  1. Department of Molecular Biology and Genetic, Faculty of Life Sciences, Muğla Sıtkı Koçman University, 48000, Muğla, Turkey

    Ömür Baysal

  2. Batı Akdeniz Agricultural Research Institute, 07100, Antalya, Turkey

    İlknur Polat

  3. Research Division Palermo, Institute of Biosciences and Bioresources (IBBR) – CNR, Corso Calatafimi 414, 90129, Palermo, Italy

    Francesco Mercati & Francesco Carimi

  4. The Mina and Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat-Gan, Israel

    Yigal Cohen

  5. Department of Botany, Faculty of Science, Palacky University in Olomouc, Slechtitelu 11, 783 71, Olomouc, Czech Republic

    Miloslav Kitner & Ales Lebeda

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  1. İlknur Polat
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  2. Ömür Baysal
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  3. Francesco Mercati
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  4. Miloslav Kitner
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  5. Yigal Cohen
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  7. Francesco Carimi
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Correspondence to Ömür Baysal.

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İlknur Polat, Ömür Baysal, and Francesco Mercati have equal contributions on the studies.

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Polat, İ., Baysal, Ö., Mercati, F. et al. Characterization of Pseudoperonospora cubensis isolates from Europe and Asia using ISSR and SRAP molecular markers. Eur J Plant Pathol 139, 641–653 (2014). https://doi.org/10.1007/s10658-014-0420-y

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