European Journal of Plant Pathology

, Volume 131, Issue 1, pp 39–48

Diversity in genetic structure and chemotype composition of Fusarium graminearum sensu stricto populations causing wheat head blight in individual fields in Germany

Authors

  • Firas Talas
    • State Plant Breeding Institute (720)Universität Hohenheim
    • National Commission of Biotechnology (NCBT)
  • Heiko K. Parzies
    • Institute of Plant Breeding, Seed Science and Population GeneticsUniversität Hohenheim (350)
    • State Plant Breeding Institute (720)Universität Hohenheim
Original Research

DOI: 10.1007/s10658-011-9785-3

Cite this article as:
Talas, F., Parzies, H.K. & Miedaner, T. Eur J Plant Pathol (2011) 131: 39. doi:10.1007/s10658-011-9785-3

Abstract

Fusarium head blight (FHB) is one of the most destructive diseases of wheat. Twelve small commercial wheat fields (size 1–3 hectares) were sampled in Germany for Fusarium populations at three spots per field with 10 heads each. PCR assays using generic primers confirmed 338 isolates as F.graminearum sensu stricto (s.s.) (64.9%) out of 521 Fusarium spp. that were further analyzed. Populations of F. graminearum s.s. in Germany contain three types of trichothecenes with a dominancy of 15-acetyldeoxynivalenol chemotype (92%) followed by 3-acetyldeoxynivalenol chemotype (6.8%) and a few isolates of nivalenol chemotype (1.2%). All these isolates were genotyped using 19 microsatellite loci. The 12 populations showed a high genetic diversity within the small scale sampling areas resulting in 300 different haplotypes. Genetic diversity within populations (71.2%) was considerably higher than among populations (28.8%) as shown by analysis of molecular variance. Gene flow (Nm) between populations ranged from 0.76–3.16. Composition of haplotypes of one population followed over 2 years changed considerably. No correlation between genetic and geographical distance was found. In conclusion, populations of F. graminearum s.s. in Germany display a tremendous genetic variation on a local scale with a restricted diversity among populations.

Keywords

DON Fusarium head blight Mycotoxin Gene flow Population genetics

Supplementary material

10658_2011_9785_MOESM1_ESM.docx (27 kb)
Table S1 Name, use and sequence of oligonucleotides and the product size (DOCX 27.4 kb)

Copyright information

© KNPV 2011