European Journal of Plant Pathology

, Volume 130, Issue 4, pp 449–456

Tomato necrotic ring virus (TNRV), a recently described tospovirus species infecting tomato and pepper in Thailand

  • Afshin Hassani-Mehraban
  • Sirirat Cheewachaiwit
  • Cherry Relevante
  • Richard Kormelink
  • Dick Peters
Article

DOI: 10.1007/s10658-011-9771-9

Cite this article as:
Hassani-Mehraban, A., Cheewachaiwit, S., Relevante, C. et al. Eur J Plant Pathol (2011) 130: 449. doi:10.1007/s10658-011-9771-9
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Abstract

Two tospovirus isolates collected from tomato and bell pepper in Thailand were studied. The isolates induced severe necrotic mottling and/or necrotic spots and rings on the leaves and fruits of the respective plants as confirmed by back-inoculation. A polyclonal antiserum raised against its nucleocapsid (N) protein reacted only with an extract from plants infected with the homologous virus. Analysis of the nucleocapsid (N) gene sequence and its deduced amino acid sequence (Mw ∼31 kDa) showed 99% amino acid sequence homology with that of Tomato necrotic ring virus (TNRV). The nucleotide sequence of the 5΄ untranslated region and intergenic region flanking the N gene revealed typical features of the S RNA segment of tospoviruses. Mechanical inoculation of the virus on some plant species showed that most of the tested solanaceous species were susceptible to this virus. The biological, serological and molecular data presented here indicate that both isolates are identical to TNRV, a recently described tospovirus species in Thailand.

Keywords

BunyaviridaePhylogenySerological relationship

Copyright information

© KNPV 2011

Authors and Affiliations

  • Afshin Hassani-Mehraban
    • 1
  • Sirirat Cheewachaiwit
    • 2
  • Cherry Relevante
    • 3
  • Richard Kormelink
    • 1
  • Dick Peters
    • 1
  1. 1.Laboratory of Virology, Department of Plant SciencesWageningen UniversityWageningenthe Netherlands
  2. 2.Plant Pathology DepartmentHortigenetics Research, East–West Seed CompanyChiang MaiThailand
  3. 3.Plant Pathology Department, Research and DevelopmentEast–West Seed CompanyBulacanthe Philippines