European Journal of Plant Pathology

, Volume 124, Issue 2, pp 303–308

Identification of phytotoxins from Botryosphaeria obtusa, a pathogen of black dead arm disease of grapevine

  • Jules Désiré Djoukeng
  • Suzanna Polli
  • Philippe Larignon
  • Eliane Abou-Mansour
Article

DOI: 10.1007/s10658-008-9419-6

Cite this article as:
Djoukeng, J.D., Polli, S., Larignon, P. et al. Eur J Plant Pathol (2009) 124: 303. doi:10.1007/s10658-008-9419-6

Abstract

A bioassay-guided fractionation of a culture filtrate of Botryosphaeria obtusa led to the isolation of four dihydroisocoumarins, named mellein 1, 4-hydroxymellein 2, 7-hydroxymellein 3 and the new 4,7-dihydroxymellein 4. LC-UV-DAD-MS analysis of vine wood infected by B. obtusa revealed the presence of mellein (1). Botryosphaeria obtusa was also able to oxidise wood δ-resveratrol into the dimer delta-viniferin. The structures of isolated phytotoxins were established on the basis of IR, MS, 1D and 2D NMR.

Keywords

Black dead armBotryosphaeria obtusaGrapevine diseaseIsocoumarinsMelleinsPhytotoxins

Copyright information

© KNPV 2009

Authors and Affiliations

  • Jules Désiré Djoukeng
    • 1
  • Suzanna Polli
    • 1
  • Philippe Larignon
    • 2
  • Eliane Abou-Mansour
    • 1
    • 3
  1. 1.Institute of BiologyUniversity of NeuchâtelNeuchâtelSwitzerland
  2. 2.Institut Français de la Vigne et du Vin, Unité Rhône-MéditerranéeDomaine de DonadilleRodilhanFrance
  3. 3.Plant Biology DepartmentUniversity of FribourgFribourgSwitzerland