Identification of phytotoxins from Botryosphaeria obtusa, a pathogen of black dead arm disease of grapevine
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- Djoukeng, J.D., Polli, S., Larignon, P. et al. Eur J Plant Pathol (2009) 124: 303. doi:10.1007/s10658-008-9419-6
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A bioassay-guided fractionation of a culture filtrate of Botryosphaeria obtusa led to the isolation of four dihydroisocoumarins, named mellein 1, 4-hydroxymellein 2, 7-hydroxymellein 3 and the new 4,7-dihydroxymellein 4. LC-UV-DAD-MS analysis of vine wood infected by B. obtusa revealed the presence of mellein (1). Botryosphaeria obtusa was also able to oxidise wood δ-resveratrol into the dimer delta-viniferin. The structures of isolated phytotoxins were established on the basis of IR, MS, 1D and 2D NMR.