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Oxidative stress biomarkers and heart function in bullfrog tadpoles exposed to Roundup Original®

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Abstract

Oxidative stress biomarkers, in vivo heart rate (f H), and contraction dynamics of ventricle strips of bullfrog (Lithobates catesbeiana) tadpoles were evaluated after 48 h of exposure to a sub-lethal concentration (1 ppm) of the herbicide Roundup Original® (glyphosate 41%). The activities of the antioxidant enzymes superoxide dismutase and catalase were increased in the liver and decreased in muscle, while oxidative damage to lipids increased above control values in both tissues, showing that the generation of reactive oxygen species and oxidative stress are involved in the toxicity induced by Roundup®. Additionally, tadpoles’ hyperactivity was associated with tachycardia in vivo, probably due to a stress-induced adrenergic stimulation. Ventricle strips of Roundup®-exposed tadpoles (R-group) presented a faster relaxation and also a higher cardiac pumping capacity at the in vivo contraction frequency, indicating that bullfrog tadpoles were able to perform cardiac mechanistic adjustments to face Roundup®-exposure. However, the lower maximal in vitro contraction frequency of the R-group could limit its in vivo cardiac performance, when the adrenergic-stimulation is present. The association between the high energetic cost to counteract the harmful effects of this herbicide and the induction of oxidative stress suggest that low and realistic concentrations of Roundup® can have an impact on tadpoles’ performance and success, jeopardizing their survival and/or population establishment.

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Acknowledgments

Bullfrog tadpoles were kindly provided by the Estrela breeding colony at Ibaté, São Paulo State, Brazil. The authors are thankful to the field technician Mr. Angelo Carnelosi for caring for the tadpoles in the laboratory. All the experiments were performed complying with the Brazilian laws.

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  1. Campus of Sorocaba, Federal University of São Carlos, Avenida Darci Carvalho Dafferner 200, Sorocaba, SP, 18043-970, Brazil

    Monica J. Costa

  2. Department of Physiological Sciences, Federal University of São Carlos, Via Washington Luiz, km 235, Sao Carlos, SP, 13565-905, Brazil

    Diana A. Monteiro, Francisco T. Rantin & Ana L. Kalinin

  3. Center of High Technological Education, Campinas State University, Rua Paschoal Marmo, 1888, Limeira, SP, 13484-970, Brazil

    Abilio L. Oliveira-Neto

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  1. Monica J. Costa
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Correspondence to Monica J. Costa.

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Costa, M.J., Monteiro, D.A., Oliveira-Neto, A.L. et al. Oxidative stress biomarkers and heart function in bullfrog tadpoles exposed to Roundup Original® . Ecotoxicology 17, 153–163 (2008). https://doi.org/10.1007/s10646-007-0178-5

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