PRECLINICAL STUDIES

Investigational New Drugs

, Volume 31, Issue 4, pp 801-811

Therapeutic efficacy of 188Re-liposome in a C26 murine colon carcinoma solid tumor model

  • Ya-Jen ChangAffiliated withInstitute of Nuclear Energy Research
  • , Chin-Wei HsuAffiliated withInstitute of Nuclear Energy Research
  • , Chih-Hsien ChangAffiliated withInstitute of Nuclear Energy Research
  • , Keng-Li LanAffiliated withCancer Center, Taipei Veterans General HospitalDepartment of Biomedical Imaging and Radiological Sciences, School of Biomedical Science and Engineering, National Yang-Ming University
  • , Gann TingAffiliated withNational Health Research Institutes
  • , Te-Wei LeeAffiliated withInstitute of Nuclear Energy Research Email author 

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Summary

Nanoliposomes are good drug delivery systems that allow the encapsulation of drugs into vesicles for their delivery. The objective of this study is to investigate the therapeutic efficacy of a new radio-therapeutics of 188Re-labeled pegylated liposome in a C26 murine colon carcinoma solid tumor model. The safety of 188Re-liposome was evaluated before radiotherapy treatment. The anti-tumor effect of 188Re-liposome was assessed by tumor growth inhibition, survival ratio and ultrasound imaging. Apoptotic marker in tumor was also evaluated by the TUNEL (terminal deoxynucleotidyl transferase biotin-dUTP nick-end labeling) method after injection of 188Re-liposome. The group treated with 188Re-liposome displayed slight loss in body weight and decrease in white blood cell (WBC) count 7 to 14 days post-injection. With respect to therapeutic efficacy, the tumor-bearing mice treated with 188Re-liposome showed better mean tumor growth inhibition rate (MGI) and longer median survival time (MGI = 0.140; 80 day) than those treated with anti-cancer drug 5-FU (MGI = 0.195; 69 day) and untreated control mice (MGI = 0.413; 48 day). The ultrasound imaging showed a decrease in both tumor volume and number of blood vessels. There were significantly more apoptotic nuclei (TUNEL-positive) in 188Re-liposome-treated mice at 8 h after treatment than in control mice. These results evidenced the potential benefits achieved by oncological application of the radio-therapeutics 188Re-liposome for adjuvant cancer treatment.

Keywords

5-fluorouracil Liposomes Rhenium-188 Colon cancer Radiotherapy