Investigational New Drugs

, Volume 29, Issue 6, pp 1156–1163

Dequalinium induces apoptosis in peripheral blood mononuclear cells isolated from human chronic lymphocytic leukemia

Authors

  • Lucia Pajuelo
    • Servicio de InmunologíaHospital General Universitario Gregorio Marañón
  • Eva Calviño
    • Departamento de Bioquímica y Biología Molecular, Facultad de MedicinaUniversidad de Alcalá
  • Jose Carlos Diez
    • Departamento de Bioquímica y Biología Molecular, Facultad de MedicinaUniversidad de Alcalá
  • Maria del Carmen Boyano-Adánez
    • Departamento de Bioquímica y Biología Molecular, Facultad de MedicinaUniversidad de Alcalá
  • Juana Gil
    • Servicio de InmunologíaHospital General Universitario Gregorio Marañón
    • Departamento de Bioquímica y Biología Molecular, Facultad de MedicinaUniversidad de Alcalá
PRECLINICAL STUDIES

DOI: 10.1007/s10637-010-9454-y

Cite this article as:
Pajuelo, L., Calviño, E., Diez, J.C. et al. Invest New Drugs (2011) 29: 1156. doi:10.1007/s10637-010-9454-y

Summary

B-cell chronic lymphocytic leukemia (B-CLL) is an abnormal neoplasic proliferation of B cells, which accumulate mainly in the bone marrow and blood preventing both B cells development in the lymph nodes and the ability to fight against infection. The antitumor agents used in chemotherapy are aimed at inducing malignant cell death, thus limiting the growth and spreading of these cells. However, the lack of specificity for tumor cells exhibited by these agents causes undesirable side effects that have led to the investigation of new therapeutic strategies designed to specifically target malignant cells and thus trigger selective cell destruction. Dequalinium (DQA) is an antitumoral agent that selectively accumulates in the mitochondria and has been shown to display anticancer activity in cells from different malignancies. In the present study, the DQA-induced cytotoxicity in B-CLL cells was analyzed by measuring cell viability and cell death, either by necrosis or apoptosis. Our results support the importance of DQA as a selective and potential antileukemic drug with a higher cytotoxic effect on peripheral blood mononuclear cells from B-CLL patients than in those from healthy donors and encourage the performance of further studies in combination with other agents.

Keywords

Chronic lymphocytic leukemiaDequaliniumApoptosisNecrosisCell deathAntitumoral agents

Copyright information

© Springer Science+Business Media, LLC 2010