Investigational New Drugs

, Volume 27, Issue 3, pp 214–222

Antioxidant and anticancer efficacy of hesperidin in benzo(a)pyrene induced lung carcinogenesis in mice

  • Sattu Kamaraj
  • Gopalakrishnan Ramakrishnan
  • Pandi Anandakumar
  • Sundaram Jagan
  • Thiruvengadam Devaki
PRECLINICAL STUDIES

DOI: 10.1007/s10637-008-9159-7

Cite this article as:
Kamaraj, S., Ramakrishnan, G., Anandakumar, P. et al. Invest New Drugs (2009) 27: 214. doi:10.1007/s10637-008-9159-7

Summary

Chemoprevention is regarded as one of the most promising and realistic approaches in the prevention of cancer. Several bioactive compounds present in fruits and vegetables have revealed their cancer curative potential on lung cancer. Hesperidin is one such naturally occurring flavonoid widely found in citrus fruits. The aim of the present study is to divulge the chemopreventive nature of hesperidin during benzo(a)pyrene (B(a)P) induced lung cancer in Swiss albino mice. Administration of B(a)P (50 mg/kg body weight) to mice resulted in increased lipid peroxides (LPO), lung specific tumor marker carcinoembryonic antigen (CEA) and serum marker enzymes aryl hydrocarbon hydroxylase (AHH), gamma glutamyl transpeptidase (GGT), 5′nucleotidase (5′ND) and lactate dehydrogenase (LDH) with concomitant decrease in the levels of tissue antioxidants like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), vitamin E and vitamin C. Hesperidin supplementation (25 mg/kg body weight) significantly attenuated these alterations thereby showing potent anticancer effect in lung cancer. Further the antiproliferative effect of hesperidin was confirmed by histopathological analysis and proliferating cell nuclear antigen (PCNA) immunostaining. Overall, these findings substantiate the chemopreventive potential of hesperidin against chemically induced lung cancer in mice.

Keywords

Lung cancerHesperidinLPO and antioxidantsTumor markersPCNA

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Sattu Kamaraj
    • 1
  • Gopalakrishnan Ramakrishnan
    • 1
  • Pandi Anandakumar
    • 1
  • Sundaram Jagan
    • 1
  • Thiruvengadam Devaki
    • 1
  1. 1.Department of BiochemistryUniversity of MadrasChennaiIndia