Documenta Ophthalmologica

, Volume 128, Issue 3, pp 155–168

Contribution of retinal ganglion cells to the mouse electroretinogram

Authors

  • Benjamin J. Smith
    • Department of BiologyDalhousie University
  • Xu Wang
    • Retina and Optic Nerve Research LaboratoryDalhousie University
    • Department of Physiology and BiophysicsDalhousie University
    • Unit on Neuron-Glia Interactions in Retinal Disease, National Eye InstituteNational Institutes of Health
  • Balwantray C. Chauhan
    • Retina and Optic Nerve Research LaboratoryDalhousie University
    • Department of Physiology and BiophysicsDalhousie University
    • Department of Ophthalmology and Visual SciencesDalhousie University
    • Department of BiologyDalhousie University
    • Department of Ophthalmology and Visual SciencesDalhousie University
  • François Tremblay
    • Retina and Optic Nerve Research LaboratoryDalhousie University
    • Department of Physiology and BiophysicsDalhousie University
    • Department of Ophthalmology and Visual SciencesDalhousie University
Original Research Article

DOI: 10.1007/s10633-014-9433-2

Cite this article as:
Smith, B.J., Wang, X., Chauhan, B.C. et al. Doc Ophthalmol (2014) 128: 155. doi:10.1007/s10633-014-9433-2

Abstract

Purpose

To quantify the direct contribution of retinal ganglion cells (RGCs) on individual components of the mouse electroretinogram (ERG).

Methods

Dark- and light-adapted ERGs from mice 8 to 12 weeks after optic nerve transection (ONTx, n = 14) were analyzed through stimulus response curves for a- and b-waves, oscillatory potentials (OPs), positive and negative scotopic threshold response (p/n STR), and the photopic negative response (PhNR) and compared with unoperated and sham-operated controls, as well as to eyes treated with 6-cyano-7-nitroquinoxaline-2,3-dion (CNQX).

Results

We confirmed in mice that CNQX intravitreal injection reduced the scotopic a-wave amplitude at high flash strength, confirming a post-receptoral contribution to the a-wave. We found that ONTx, which is more specific to RGCs, did not affect the a-wave amplitude and implicit time in either photopic or scotopic conditions while the b-wave was reduced. Both the pSTR and nSTR components were reduced in amplitude, with the balance between the two components resulting in a shortening of the nSTR peak implicit time. On the other hand, amplitude of the PhNR was increased while the OPs were minimally affected.

Conclusion

With an intact a-wave demonstrated following ONTx, we find that the most robust indicators of RGC function in the mouse full-field ERG were the STR components.

Keywords

Retinal ganglion cellsOptic nerveAxotomyElectroretinogramMouseScotopic threshold responsePhotopic negative response

Copyright information

© Springer-Verlag Berlin Heidelberg 2014