Documenta Ophthalmologica

, Volume 128, Issue 3, pp 155–168

Contribution of retinal ganglion cells to the mouse electroretinogram

  • Benjamin J. Smith
  • Xu Wang
  • Balwantray C. Chauhan
  • Patrice D. Côté
  • François Tremblay
Original Research Article

DOI: 10.1007/s10633-014-9433-2

Cite this article as:
Smith, B.J., Wang, X., Chauhan, B.C. et al. Doc Ophthalmol (2014) 128: 155. doi:10.1007/s10633-014-9433-2

Abstract

Purpose

To quantify the direct contribution of retinal ganglion cells (RGCs) on individual components of the mouse electroretinogram (ERG).

Methods

Dark- and light-adapted ERGs from mice 8 to 12 weeks after optic nerve transection (ONTx, n = 14) were analyzed through stimulus response curves for a- and b-waves, oscillatory potentials (OPs), positive and negative scotopic threshold response (p/n STR), and the photopic negative response (PhNR) and compared with unoperated and sham-operated controls, as well as to eyes treated with 6-cyano-7-nitroquinoxaline-2,3-dion (CNQX).

Results

We confirmed in mice that CNQX intravitreal injection reduced the scotopic a-wave amplitude at high flash strength, confirming a post-receptoral contribution to the a-wave. We found that ONTx, which is more specific to RGCs, did not affect the a-wave amplitude and implicit time in either photopic or scotopic conditions while the b-wave was reduced. Both the pSTR and nSTR components were reduced in amplitude, with the balance between the two components resulting in a shortening of the nSTR peak implicit time. On the other hand, amplitude of the PhNR was increased while the OPs were minimally affected.

Conclusion

With an intact a-wave demonstrated following ONTx, we find that the most robust indicators of RGC function in the mouse full-field ERG were the STR components.

Keywords

Retinal ganglion cellsOptic nerveAxotomyElectroretinogramMouseScotopic threshold responsePhotopic negative response

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Benjamin J. Smith
    • 1
  • Xu Wang
    • 2
    • 3
    • 5
  • Balwantray C. Chauhan
    • 2
    • 3
    • 4
  • Patrice D. Côté
    • 1
    • 4
  • François Tremblay
    • 2
    • 3
    • 4
  1. 1.Department of BiologyDalhousie UniversityHalifaxCanada
  2. 2.Retina and Optic Nerve Research LaboratoryDalhousie UniversityHalifaxCanada
  3. 3.Department of Physiology and BiophysicsDalhousie UniversityHalifaxCanada
  4. 4.Department of Ophthalmology and Visual SciencesDalhousie UniversityHalifaxCanada
  5. 5.Unit on Neuron-Glia Interactions in Retinal Disease, National Eye InstituteNational Institutes of HealthBethesdaCanada