Contribution of retinal ganglion cells to the mouse electroretinogram
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To quantify the direct contribution of retinal ganglion cells (RGCs) on individual components of the mouse electroretinogram (ERG).
Dark- and light-adapted ERGs from mice 8 to 12 weeks after optic nerve transection (ONTx, n = 14) were analyzed through stimulus response curves for a- and b-waves, oscillatory potentials (OPs), positive and negative scotopic threshold response (p/n STR), and the photopic negative response (PhNR) and compared with unoperated and sham-operated controls, as well as to eyes treated with 6-cyano-7-nitroquinoxaline-2,3-dion (CNQX).
We confirmed in mice that CNQX intravitreal injection reduced the scotopic a-wave amplitude at high flash strength, confirming a post-receptoral contribution to the a-wave. We found that ONTx, which is more specific to RGCs, did not affect the a-wave amplitude and implicit time in either photopic or scotopic conditions while the b-wave was reduced. Both the pSTR and nSTR components were reduced in amplitude, with the balance between the two components resulting in a shortening of the nSTR peak implicit time. On the other hand, amplitude of the PhNR was increased while the OPs were minimally affected.
With an intact a-wave demonstrated following ONTx, we find that the most robust indicators of RGC function in the mouse full-field ERG were the STR components.
- Contribution of retinal ganglion cells to the mouse electroretinogram
Volume 128, Issue 3 , pp 155-168
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- Springer Berlin Heidelberg
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- Retinal ganglion cells
- Optic nerve
- Scotopic threshold response
- Photopic negative response
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- Author Affiliations
- 1. Department of Biology, Dalhousie University, PO Box 15000, Halifax, NS, B3H 4R2, Canada
- 2. Retina and Optic Nerve Research Laboratory, Dalhousie University, PO Box 15000, Halifax, NS, B3H 4R2, Canada
- 3. Department of Physiology and Biophysics, Dalhousie University, PO Box 15000, Halifax, NS, B3H 4R2, Canada
- 5. Unit on Neuron-Glia Interactions in Retinal Disease, National Eye Institute, National Institutes of Health, 6 Center Drive, 6/215, Bethesda, MD, 20892, Canada
- 4. Department of Ophthalmology and Visual Sciences, Dalhousie University, 1276 South Park Street, Halifax, NS, B3H 2Y9, Canada