Digestive Diseases and Sciences

, Volume 58, Issue 1, pp 140–149

H. pylori Infection Is Associated with DNA Damage of Lgr5-Positive Epithelial Stem Cells in the Stomach of Patients with Gastric Cancer

  • Takeshi Uehara
  • Deqin Ma
  • Yuan Yao
  • John P. Lynch
  • Knashawn Morales
  • Amy Ziober
  • Michael Feldman
  • Hiroyoshi Ota
  • Antonia R. Sepulveda
Original Article

DOI: 10.1007/s10620-012-2360-8

Cite this article as:
Uehara, T., Ma, D., Yao, Y. et al. Dig Dis Sci (2013) 58: 140. doi:10.1007/s10620-012-2360-8

Abstract

Background

H. pylori (Hp) infection is a major risk factor in gastric carcinogenesis leading to epithelial mutagenesis, and may affect gastric epithelial stem cells.

Aims

To characterize the expression of Lgr5, a marker of epithelial stem cells in human gastric mucosa, to determine whether Hp infection affects Lgr5-positive epithelial cells (LPECs) and whether LPECs are susceptible to DNA damage associated with Hp infection.

Methods

Lgr5 expression was characterized in non-neoplastic gastric mucosa from 52 patients (34 with and 18 without gastric cancer (GC); 21 Hp-positive (Hp+) and 31 Hp-negative (Hp−)) by immunohistochemical and immunofluorescence staining. To determine the extent of DNA damage in LPECs, nuclear 8-hydroxydeoxyguanosine (8OHdG), a marker of DNA damage associated with oxidative stress, was measured by quantitative spectral image analysis.

Results

LPECs were primarily present in gastric antrum. Higher numbers of LPECs were seen in Hp+ than in Hp− non-neoplastic mucosa of GC patients, P = .006, but not in patients without GC. 8OHdG levels in LPECs were significantly higher than in Lgr5-negative epithelial cells in Hp+ GC patients (P = .012) but not in Hp− cases (P = .414), whereas no difference was seen between Hp+ and Hp− mucosa of patients without GC.

Conclusions

The Lgr5-positive epithelial stem cell pool is expanded in Hp-associated gastritis in the antrum of patients with GC. In GC patients with active Hp infection, LPECs may be more susceptible to DNA damage than Lgr5-negative epithelial cells, suggesting that Hp infection may contribute to GC risk by affecting epithelial stem cells in the human stomach.

Keywords

Helicobacter pyloriGastricDNA damage8OHdGLgr5Stem cells

Supplementary material

10620_2012_2360_MOESM1_ESM.docx (29 kb)
Supplementary material 1 (DOCX 29 kb)
10620_2012_2360_MOESM2_ESM.tif (9.8 mb)
Supplementary material 2 (TIFF 10023 kb)
10620_2012_2360_MOESM3_ESM.tif (24.5 mb)
Supplementary material 3 (TIFF 25119 kb)
10620_2012_2360_MOESM4_ESM.tif (24.8 mb)
Supplementary material 4 (TIFF 25390 kb)
10620_2012_2360_MOESM5_ESM.tif (6.7 mb)
Supplementary material 5 (TIFF 6833 kb)
10620_2012_2360_MOESM6_ESM.tif (8 mb)
Supplementary material 6 (TIFF 8158 kb)
10620_2012_2360_MOESM7_ESM.tif (7 mb)
Supplementary material 7 (TIFF 7180 kb)
10620_2012_2360_MOESM8_ESM.tif (31.6 mb)
Supplementary material 8 (TIFF 32346 kb)
10620_2012_2360_MOESM9_ESM.docx (35 kb)
Supplementary material 9 (DOCX 35 kb)

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Takeshi Uehara
    • 1
    • 2
  • Deqin Ma
    • 1
  • Yuan Yao
    • 1
  • John P. Lynch
    • 3
  • Knashawn Morales
    • 4
  • Amy Ziober
    • 1
  • Michael Feldman
    • 1
  • Hiroyoshi Ota
    • 2
  • Antonia R. Sepulveda
    • 1
  1. 1.Department of Pathology and Laboratory MedicineUniversity of Pennsylvania School of MedicinePhiladelphiaUSA
  2. 2.Department of Laboratory MedicineShinshu University School of MedicineMatsumotoJapan
  3. 3.Division of Gastroenterology, Department of MedicineUniversity of Pennsylvania School of MedicinePhiladelphiaUSA
  4. 4.Department of BiostatisticsUniversity of Pennsylvania School of MedicinePhiladelphiaUSA