Adiponectin and Plant-Derived Mammalian Adiponectin Homolog Exert a Protective Effect in Murine Colitis
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Hypoadiponectinemia has been associated with states of chronic inflammation in humans. Mesenteric fat hypertrophy and low adiponectin have been described in patients with Crohn’s disease. We investigated whether adiponectin and the plant-derived homolog, osmotin, are beneficial in a murine model of colitis.
C57BL/6 mice were injected (i.v.) with an adenoviral construct encoding the full-length murine adiponectin gene (AN+DSS) or a reporter—LacZ (Ctr and V+DSS groups) prior to DSS colitis protocol. In another experiment, mice with DSS colitis received either osmotin (Osm+DSS) or saline (DSS) via osmotic pumps. Disease progression and severity were evaluated using body weight, stool consistency, rectal bleeding, colon lengths, and histology. In vitro experiments were carried out in bone marrow-derived dendritic cells.
Mice overexpressing adiponectin had lower expression of proinflammatory cytokines (TNF, IL-1β), adipokines (angiotensin, osteopontin), and cellular stress and apoptosis markers. These mice had higher levels of IL-10, alternative macrophage marker, arginase 1, and leukoprotease inhibitor. The plant adiponectin homolog osmotin similarly improved colitis outcome and induced robust IL-10 secretion. LPS induced a state of adiponectin resistance in dendritic cells that was reversed by treatment with PPARγ agonist and retinoic acid.
Adiponectin exerted protective effects during murine DSS colitis. It had a broad activity that encompassed cytokines, chemotactic factors as well as processes that assure cell viability during stressful conditions. Reducing adiponectin resistance or using plant-derived adiponectin homologs may become therapeutic options in inflammatory bowel disease.
- Adiponectin and Plant-Derived Mammalian Adiponectin Homolog Exert a Protective Effect in Murine Colitis
Digestive Diseases and Sciences
Volume 56, Issue 10 , pp 2818-2832
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- Online ISSN
- Springer US
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- Inflammatory bowel disease
- Mammalian adiponectin homolog
- PPARγ agonists
- Dendritic cells
- Inflammatory bowel disease
- Industry Sectors
- Author Affiliations
- 1. Division of Digestive Diseases and Nutrition, Microbiology, Immunology and Molecular Genetics, University of Kentucky Medical Center, 800 Rose Street, Room MN 649, Lexington, KY, 40536, USA
- 2. Graduate Center for Nutritional Sciences, University of Kentucky, Lexington, KY, 40536, USA
- 3. Plant Stress Genomics Research Center, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Kingdom of Saudi Arabia
- 4. Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY, 40536, USA
- 6. Division of Applied Sciences, WCU program, Gyeongsang National University, Jinju, 660-701, South Korea
- 7. Departments of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN, 47907, USA
- 5. Research Center of Cardiovascular Biology, University of Genoa, 16126, Liguria, Italy