Digestive Diseases and Sciences

, Volume 56, Issue 7, pp 1962–1966

Antibiotic Prophylaxis Prevents the Development of a Post-Infectious Phenotype in a New Rat Model of Post-Infectious IBS

Authors

    • GI Motility Program, Division of GastroenterologyCedars-Sinai Medical Center
  • Walter Morales
    • GI Motility Program, Division of GastroenterologyCedars-Sinai Medical Center
  • Sam-Ryong Jee
    • GI Motility Program, Division of GastroenterologyCedars-Sinai Medical Center
  • Kimberly Low
    • GI Motility Program, Division of GastroenterologyCedars-Sinai Medical Center
  • Laura Hwang
    • GI Motility Program, Division of GastroenterologyCedars-Sinai Medical Center
  • Venkata Pokkunuri
    • GI Motility Program, Division of GastroenterologyCedars-Sinai Medical Center
  • Jim Mirocha
    • GI Motility Program, Division of GastroenterologyCedars-Sinai Medical Center
  • Jeffrey Conklin
    • GI Motility Program, Division of GastroenterologyCedars-Sinai Medical Center
  • Christopher Chang
    • GI Motility Program, Division of GastroenterologyCedars-Sinai Medical Center
Original Article

DOI: 10.1007/s10620-010-1548-z

Cite this article as:
Pimentel, M., Morales, W., Jee, S. et al. Dig Dis Sci (2011) 56: 1962. doi:10.1007/s10620-010-1548-z

Abstract

Background

A recent post-infectious rat model with Campylobacter jejuni 81-176 has replicated the events noted in humans with post-infectious irritable bowel syndrome (IBS). In this study, we test whether prophylactic treatment with the antibiotic rifaximin will prevent the development of long-term altered bowel function in this model.

Methods

Sprague–Dawley rats were divided into two groups. Both groups were gavaged with a 1 mL solution of 108 cfu/mL of C. jejuni. However, one group was also prophylactically gavaged with a solution of rifaximin 200 mg per day for 3 days (the day before gavage, the day of gavage, and the day after gavage with C. jejuni). Fresh stool was collected from rats daily until two consecutive stool cultures were negative for C. jejuni. The rats were then housed for 3 months. At the end of 3 months, fresh stool was collected on three consecutive days to determine stool % wet weight and stool consistency on a stool score.

Results

Rats that received rifaximin antibiotic prophylaxis had a greater rate of stool shedding of C. jejuni. However, the mean duration of colonization was shorter in the rifaximin-treated group (10.3 ± 7.1 days) compared to rats receiving no prophylaxis (12.6 ± 5.9 days) (P < 0.01). After 3 months, rats that did not receive rifaximin had a greater variability in stool % wet weight (P < 0.01). Furthermore, the average stool consistency over 3 days of measurement was closer to normal in the rifaximin-treated rats, with a consistency of 1.1 ± 0.3, compared to 1.5 ± 0.4 in rats receiving no prophylaxis (P < 0.00001).

Conclusions

Prophylactic treatment of rats with the antibiotic rifaximin in a new animal model of post-infectious IBS with C. jejuni mitigated the development of long-term altered stool form and function.

Keywords

Post-infectious irritable bowel syndromeRat modelRifaximin

Copyright information

© Springer Science+Business Media, LLC 2011