Digestive Diseases and Sciences

, Volume 56, Issue 3, pp 721–730

Protective Effects of Black Cumin (Nigella sativa) Oil on TNBS-Induced Experimental Colitis in Rats

  • F. Isik
  • Tugba Tunali Akbay
  • A. Yarat
  • Z. Genc
  • R. Pisiriciler
  • E. Caliskan-Ak
  • S. Cetinel
  • A. Altıntas
  • G. Sener
Original Article

DOI: 10.1007/s10620-010-1333-z

Cite this article as:
Isik, F., Tunali Akbay, T., Yarat, A. et al. Dig Dis Sci (2011) 56: 721. doi:10.1007/s10620-010-1333-z

Abstract

Background

The pathogenesis and treatment of ulcerative colitis remain poorly understood. The aim of the present study is to investigate the effects of black cumin (Nigella sativa) oil on rats with colitis.

Methods

Experimental colitis was induced with 1 mL trinitrobenzene sulfonic acid (TNBS) in 40% ethanol by intracolonic administration with 8-cm-long cannula under ether anesthesia to rats in colitis group and colitis + black cumin oil group. Rats in the control group were given saline at the same volume by intracolonic administration. Black cumin oil (BCO, Origo “100% natural Black Cumin Seed Oil,” Turkey) was given to colitis + black cumin oil group by oral administration during 3 days, 5 min after colitis induction. Saline was given to control and colitis groups at the same volume by oral administration. At the end of the experiment, macroscopic lesions were scored and the degree of oxidant damage was evaluated by colonic total protein, sialic acid, malondialdehyde, and glutathione levels, collagen content, and tissue factor, superoxide dismutase, and myeloperoxidase activities. Tissues were also examined by histological and cytological analysis. Proinflammatory cytokines [tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, and IL-6], lactate dehydrogenase activity, and triglyceride and cholesterol levels were analyzed in blood samples.

Results

We found that black cumin oil decreased the proinflammatory cytokines, lactate dehydrogenase, triglyceride, and cholesterol, which were increased in colitis.

Conclusions

BCO, by preventing inflammatory status in the blood, partly protected colonic tissue against experimental ulcerative colitis.

Keywords

ColitisNigella sativaInflammationTissue factor activityOxidant damage

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • F. Isik
    • 1
  • Tugba Tunali Akbay
    • 1
  • A. Yarat
    • 1
  • Z. Genc
    • 1
  • R. Pisiriciler
    • 2
  • E. Caliskan-Ak
    • 2
  • S. Cetinel
    • 3
  • A. Altıntas
    • 4
  • G. Sener
    • 5
  1. 1.Faculty of Dentistry, Department of BiochemistryMarmara UniversityIstanbulTurkey
  2. 2.Faculty of Dentistry, Department of Histology and EmbryologyMarmara UniversityIstanbulTurkey
  3. 3.Faculty of Medicine, Department of Histology and EmbryologyMarmara UniversityIstanbulTurkey
  4. 4.Faculty of Pharmacy, Department of PharmacognosyAnadolu UniversityEskisehirTurkey
  5. 5.Faculty of Pharmacy, Department of PharmacologyMarmara UniversityIstanbulTurkey