Digestive Diseases and Sciences

, Volume 55, Issue 8, pp 2288–2293

Association of MicroRNA-196a-2 Gene Polymorphism with Gastric Cancer Risk in a Chinese Population

Authors

    • Department of AnesthesiologyThe Fourth Affiliated Hospital of Soochow University
  • Zhongshen Kuang
    • Intensive Care Unit of the First Affiliated Hospital of Medical CollegeShihezi University
  • Chenyi Sheng
    • Department of General SurgeryAffiliated Hospital of Nantong University
  • Yan Zhang
    • Department of AnesthesiologyThe Fourth Affiliated Hospital of Soochow University
  • Hang Xu
    • Intensive Care Unit of the First Affiliated Hospital of Medical CollegeShihezi University
  • Qinghong Cheng
    • Intensive Care Unit of the First Affiliated Hospital of Medical CollegeShihezi University
Original Article

DOI: 10.1007/s10620-009-1007-x

Cite this article as:
Peng, S., Kuang, Z., Sheng, C. et al. Dig Dis Sci (2010) 55: 2288. doi:10.1007/s10620-009-1007-x

Abstract

Background

It has been proposed that single nucleotide polymorphisms (SNPs) in microRNAs (miRNAs) could affect the expression of the miRNA and contribute to the susceptibility of human tumors. However, the role of genetic variant (T/C) in miR-196a-2 in gastric cancer susceptibility is still unknown.

Objectives

To evaluate the association between genetic polymorphism of miR-196a-2 (rs11614913) and risk of gastric cancer, a hospital-based case–control study was conducted in a Chinese population.

Methods

The miR-196a-2 polymorphism was determined using the method of polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP) in 213 gastric cancer patients and 213 age- and sex-matched controls.

Results

In the present study, we found that a significantly increased risk of gastric cancer in subjects with the variant homozygote CC of miR-196a-2 compared with wild-type homozygote TT and heterozygote CT carriers (adjusted odds ratio (OR) = 1.57, 95% confidence interval (CI) = 1.03–2.39, P = 0.038). Stratified analyses indicated that the variant homozygote CC genotype had a strong association with lymph node metastasis of gastric cancer (adjusted OR = 2.25, 95% CI = 1.21–4.18, P = 0.011).

Conclusions

These findings suggest that the genetic variant within miR-196a-2 could play an important role in the development and progression of gastric cancer. We expect the findings may be helpful to better understand the mechanism of gastric carcinogenesis.

Keywords

microRNA-196a-2Gastric cancerPolymorphismCarcinogenesis

Abbreviations

CI

Confidence interval

OR

Odds ratio

PCR

Polymerase chain reaction

miRNAs

microRNAs

SNP

Single nucleotide polymorphism

Copyright information

© Springer Science+Business Media, LLC 2009