A Molecular Analysis of Fecal and Mucosal Bacterial Communities in Irritable Bowel Syndrome
Purchase on Springer.com
$39.95 / €34.95 / £29.95*
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.
The objectives of this study were, firstly, to determine the diversity of the host’s gut microbiota in irritable bowel syndrome (IBS) using a culture-independent method (DGGE of the 16S rRNA gene) and, secondly, to examine mucosal biopsies of IBS patients and compare them to their own fecal microbiota.
The diversity of the dominant microbiota in the fecal material of IBS patients was compared to a healthy control group. In addition, we compared the mucosal and fecal microbiota of IBS patients.
Statistical analysis of the mean similarity data for these groups indicated a significant difference (P < 0.001) between IBS (n = 47) and healthy controls (n = 33) with significantly more variation in the gut microbiota of healthy volunteers than that of IBS patients. The average intra-individual similarity between the mucosa and luminal microbiota was 84%, which indicates that different communities were present at the two sites. This difference, however, is similar to that previously described between these two niches in control subjects. The average inter-individual similarity of the bacterial communities on the mucosa and in the lumen of IBS was not significantly different (P > 0.05).
IBS impacts equally on both bacterial communities in the IBS host and a significant difference in the gut microbiota exists between fecal samples from IBS patients and healthy controls. The reason for this difference is unclear and various possible explanations are available, but much more work is required to determine the underlying reason for this observation.
- Agrawal A, Whorwell PJ. Irritable bowel syndrome: diagnosis and management. Br Med J. 2006;332:280–283. CrossRef
- Balsari A, Ceccarelli A, Dubini F, Fesce E, Poli G. The fecal microbial population in the irritable bowel syndrome. Microbiologica. 1982;5:185–194.
- Bayliss CE, Bradley HK, Jones VA, Hunter JO. Some aspects of colonic microbial activity in irritable bowel syndrome associated with food intolerance. Ann Ist Super Sanita. 1986;22:959–963.
- Dubini F, Balsari A. Midecamycin activity against aerobic and anaerobic bacteria. G Ital Chemioter. 1985;32:539–544.
- Eckburg PB, Bik EM, Bernstein CN, et al. Diversity of the human intestinal microbial flora. Science. 2005;308:1635–1638. CrossRef
- Green GL, Brostoff J, Hudspith B, et al. Molecular characterization of the bacteria adherent to human colorectal mucosa. J Appl Microbiol. 2006;100:460–469. CrossRef
- Kang JY. Systematic review: the influence of geography and ethnicity in irritable bowel syndrome. Aliment Pharmacol Ther. 2005;21:663–676. CrossRef
- Kassinen A, Krogius-Kurikka L, Makivuokko H, et al. The fecal microbiota of irritable bowel syndrome patients differs significantly from that of healthy subjects. Gastroenterol. 2007;133:24–33. CrossRef
- King TS, Elia M, Hunter JO. Abnormal colonic fermentation in irritable bowel syndrome. Lancet. 1998;352:1187–1189. CrossRef
- Lane DJ. 16S/23S rRNA Sequencing. In: Stackebrandt E, Goodfellow M, eds. Nucleic acids techniques in bacterial systematics. Chichester: Wiley; 1991:115–175.
- Lepage P, Seksik P, Sutren M, et al. Biodiversity of the mucosa-associated microbiota is stable along the distal digestive tract in healthy individuals and patients with IBD. Inflamm Bowel Dis. 2005;11:473–480. CrossRef
- Madden JA, Hunter JO. A review of the role of the gut microflora in irritable bowel syndrome and the effects of probiotics. Br J Nutr. 2002;88(suppl 1):S67–S72. CrossRef
- Malinen E, Rinttila T, Kajander K, et al. Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR. Am J Gastroenterol. 2005;100:373–382. CrossRef
- Mangin I, Bonnet R, Seksik P, et al. Molecular inventory of faecal microflora in patients with Crohn’s disease. FEMS Microbiol Ecol. 2004;50:25–36. CrossRef
- Manichanh C, Rigottier-Gois L, Bonnaud E, et al. Reduced diversity of faecal microbiota in Crohn’s disease revealed by a metagenomic approach. Gut. 2006;55:205–211. CrossRef
- Matto J, Maunuksela L, Kajander K, et al. Composition and temporal stability of gastrointestinal microbiota in irritable bowel syndrome—a longitudinal study in IBS and control subjects. FEMS Immunol Med Microbiol. 2005;43:213–222. CrossRef
- Maukonen J, Satokari R, Matto J, Soderlund H, Mattila-Sandholm T, Saarela M. Prevalence and temporal stability of selected clostridial groups in irritable bowel syndrome in relation to predominant faecal bacteria. J Med Microbiol. 2006;55:625–633. CrossRef
- Nandi R, Sengupta S. Microbial production of hydrogen: an overview. Crit Rev Microbiol. 1998;24:61–84. CrossRef
- Nubel U, Engelen B, Felske A, et al. Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. J Bacteriol. 1996;178:5636–5643.
- O’Mahony L, McCarthy J, Kelly P, et al. Lactobacillus and Bifidobacterium in irritable bowel syndrome: symptom responses and relationship to cytokine profiles. Gastroenterol. 2005;128:541–551. CrossRef
- Pimentel M, Chow EJ, Lin HC. Eradication of small intestinal bacterial overgrowth reduces symptoms of irritable bowel syndrome. Am J Gastroenterol. 2000;95:3503–3506. CrossRef
- Pimentel M, Chow EJ, Lin HC. Normalization of lactulose breath testing correlates with symptom improvement in irritable bowel syndrome. A double-blind, randomized, placebo-controlled study. Am J Gastroenterol. 2003;98:412–419.
- Pimentel M, Lin HC, Enayati P, et al. Methane, a gas produced by enteric bacteria, slows intestinal transit and augments small intestinal contractile activity. Am J Physiol Gastrointest Liver Physiol. 2005;290:G1089–G1095. CrossRef
- Posserud I, Stotzer PO, Bjornsson E, Abrahamsson H, Simren M. Small intestinal bacterial overgrowth in patients with irritable bowel syndrome. Gut. 2006;56:802–808. CrossRef
- Quigley EM. Changing face of irritable bowel syndrome. World J Gastroenterol. 2006;12:1–5.
- Rinttila T, Kassinen A, Malinen E, Krogius L, Palva A. Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J Appl Microbiol. 2004;97:1166–1177. CrossRef
- Savage DC. Microbial ecology of the gastrointestinal tract. Annu Rev Microbiol. 1977;31:107–133. CrossRef
- Scanlan PD, Shanahan F, O’Mahony C, Marchesi JR. Culture-independent analyses of the temporal variation of the dominant faecal microbiota and targeted bacterial sub-groups in Crohn’s disease. J Clin Microbiol. 2006;44:3980–3988. CrossRef
- Seksik P, Lepage P, de la Cochetiere MF, et al. Search for localized dysbiosis in Crohn’s disease ulcerations by temporal temperature gradient gel electrophoresis of 16S rRNA. J Clin Microbiol. 2005;43:4654–4658. CrossRef
- Shanahan F. Host-flora interactions in inflammatory bowel disease. Inflamm Bowel Dis. 2004;10:S16–S24. CrossRef
- Si JM, Yu YC, Fan YJ, Chen SJ. Intestinal microecology and quality of life in irritable bowel syndrome patients. World J Gastroenterol. 2004;10:1802–1805.
- Swidsinski A, Weber J, Loening-Baucke V, Hale LP, Lochs H. Spatial organization and composition of the mucosal flora in patients with inflammatory bowel disease. J Clin Microbiol. 2005;43:3380–3389. CrossRef
- Thompson WG, Longstreth GF, Drossman DA, Heaton KW, Irvine EJ, Muller-Lissner SA. Functional bowel disorders and functional abdominal pain. Gut. 1999;45:43ii–47.
- van de Peer Y, Chapelle S, Dewachter R. A quantitative map of nucleotide substitution rates in bacterial rRNA. Nucl Acid Res. 1996;24:3381–3391. CrossRef
- Vanhoutte T, Huys G, De Brandt E, Swings J. Temporal stability analysis of the microbiota in human feces by denaturing gradient gel electrophoresis using universal and group-specific 16S rRNA gene primers. FEMS Microbiol Ecol. 2004;48:437–446. CrossRef
- Zhang M, Liu B, Zhang Y, Wei H, Lei Y, Zhao L. Structural shifts of mucosa-associated Lactobacilli and Clostridium leptum subgroup in patients with ulcerative colitis. J Clin Microbiol. 2007;45:496–500. CrossRef
- Zoetendal EG, von Wright A, Vilpponen-Salmela T, Ben Amor K, Akkermans AD, de Vos WM. Mucosa-associated bacteria in the human gastrointestinal tract are uniformly distributed along the colon and differ from the community recovered from feces. Appl Environ Microbiol. 2002;68:3401–3407. CrossRef
- A Molecular Analysis of Fecal and Mucosal Bacterial Communities in Irritable Bowel Syndrome
Digestive Diseases and Sciences
Volume 55, Issue 2 , pp 392-397
- Cover Date
- Print ISSN
- Online ISSN
- Springer US
- Additional Links
- Irritable bowel syndrome
- Colonic microbiota
- Gut flora
- Fecal flora
- Mucosal microbiota
- Industry Sectors
- Author Affiliations
- 1. Alimentary Pharmabiotic Centre, Department of Medicine, Cork University Hospital, Cork, Ireland
- 2. Department of Microbiology, National University of Ireland, Cork, Western Road, Cork, Ireland
- 3. Department of Medicine, Cork University Hospital, Wilton, Cork, Ireland
- 4. School of Biosciences, Cardiff University, Cardiff, CF10 3AT, UK