Digestive Diseases and Sciences

, Volume 55, Issue 3, pp 709–715

Reproducibility and Performance Characteristics of Colonic Compliance, Tone, and Sensory Tests in Healthy Humans

Authors

  • Suwebatu T. Odunsi
    • Clinical Enteric Neuroscience Translational and Epidemiological Research (C.E.N.T.E.R.), College of MedicineMayo Clinic
    • Clinical Enteric Neuroscience Translational and Epidemiological Research (C.E.N.T.E.R.), College of MedicineMayo Clinic
  • Adil E. Bharucha
    • Clinical Enteric Neuroscience Translational and Epidemiological Research (C.E.N.T.E.R.), College of MedicineMayo Clinic
  • Athanasios Papathanasopoulos
    • Clinical Enteric Neuroscience Translational and Epidemiological Research (C.E.N.T.E.R.), College of MedicineMayo Clinic
  • Irene Busciglio
    • Clinical Enteric Neuroscience Translational and Epidemiological Research (C.E.N.T.E.R.), College of MedicineMayo Clinic
  • Duane Burton
    • Clinical Enteric Neuroscience Translational and Epidemiological Research (C.E.N.T.E.R.), College of MedicineMayo Clinic
  • Alan R. Zinsmeister
    • Clinical Enteric Neuroscience Translational and Epidemiological Research (C.E.N.T.E.R.), College of MedicineMayo Clinic
    • Division of Biostatistics, Department of Health Sciences Research, College of MedicineMayo Clinic
Original Article

DOI: 10.1007/s10620-009-0772-x

Cite this article as:
Odunsi, S.T., Camilleri, M., Bharucha, A.E. et al. Dig Dis Sci (2010) 55: 709. doi:10.1007/s10620-009-0772-x

Abstract

Background The performance characteristics of colonic sensation and motility measurements are unclear. Aim To compare left colon compliance, tone, and sensation in males and females and to evaluate inter and intra-individual coefficients of variation (COV) in these measurements. Methods Data were acquired using standard barostat methods, by one technologist, in 72 human volunteers (38 males, 18–65 years). We measured compliance, fasting tone, and sensation during baseline and post-placebo; postprandial (PP) tone was measured only post-placebo. Compliance and thresholds for first sensation, gas, and pain were measured using ascending method of limits; sensory ratings (0–100 mm VAS) using random phasic distensions at 8–36 mmHg above baseline operating pressure. Change in PP tone was measured by barostat balloon volume for the first 30 min after a 1000-kcal meal. Inter-COV was calculated as 100 (SD/mean), and intra-COV as (100 × SD delta/overall mean). Results There were no statistically significant associations with gender for most sensory or motor data at baseline. A modest association of fasting colonic tone and gender was observed. COV are lower (20–35%) for compliance, fasting tone, pain threshold, and sensation ratings than for PP tone and threshold for first or gas sensation (>45%). COV data are similar in males and females; sensation COVs appear smaller in females than in males. Conclusions Testing of compliance, tone and pain, and gas sensation in left colon performs adequately to assess these functions in humans. Lower COV for sensation tests among females is relevant to plan studies of drugs intended for functional GI disorders.

Keywords

Sensory ratingsAscending method of limitsPerceptionVisceralPostprandial

Introduction

Visceral hypersensitivity, or an excessive perception of visceral stimuli that are not usually painful, is considered a pathophysiological mechanism in functional bowel disorders such as the irritable bowel syndrome (IBS) [1, 2] and functional dyspepsia [3]. Visceral hypersensitivity is a target for the development of novel therapies modulating afferent neurotransmission [4]. Standardized distensions of the rectum using balloon distension with a barostat device are currently used for the identification of hypersensitivity in IBS [2, 510]. These same methods also provide assessment of sensorimotor function and are used in experimental therapy trials in health and disease [11]. In a prior two-center study, we previously documented the performance characteristics of rectal compliance and sensation studies in healthy volunteers [12]. Others have also documented the ability of the rectal barostat studies to measure rectal compliance, tone and the response to the meal [13, 14]. However, there are technical and anatomical reasons why evaluation of colonic function may be required instead of rectal function studies. These include the artifact introduced from weight of intra-abdominal organs or the presence of the uterus in close proximity to the rectum, which may impact on measurements of tone and compliance. There is also evidence that the rectum is associated with relatively blunted tone responses to agents, such as glucagon, which relax tone [14].

In the published literature, visceral sensitivity has been quantified by measuring the threshold, which is expressed as the pressure at which a sensation (such as urgency or pain or gas-like sensations) occurs, or the sensory rating using visual analog scales in response to graded distensions [11].

Our previous study evaluated the intra- and inter-individual variability of these measurements in the rectum [12]. In contrast, the performance characteristics of measurements of colonic compliance, tone, and sensation used in physiological and pharmacodynamic studies in healthy volunteers are unclear. Therefore, our aims were to compare these functions between males and females, and to evaluate intra- and inter-individual coefficient of variation (COV) of left colon compliance, tone, and sensation in humans.

Methods

Study Design and Participants

This study was based on data acquired in different pharmacodynamic studies using standard barostat methods using a rigid piston device and conducted by one technologist (I.B.), in 72 human volunteers (38 males, aged 18–65 years). All participants signed informed consent in the respective studies. In all of the studies, concurrent co-morbidities, abdominal symptoms, previous abdominal surgery and concomitant somatization or psychological disorders were excluded by means of a validated bowel symptom questionnaire, a somatic symptom checklist, and a physical exam [15]. The study, based on the combined database, was approved by the Mayo Clinic Institutional Review Board.

When the studies were conducted, treatment was double-blinded. Subsequently, the data from participants randomized to placebo were included in this study. All participants underwent examinations of compliance, fasting tone, and sensation during baseline and post-placebo; postprandial tone was measured only post-placebo.

The experimental design is summarized in Fig. 1. Intubation was achieved with unsedated, limited colonoscopy and fluoroscopy. All methods used in this study have been described in detail elsewhere and are summarized briefly here.
https://static-content.springer.com/image/art%3A10.1007%2Fs10620-009-0772-x/MediaObjects/10620_2009_772_Fig1_HTML.gif
Fig. 1

Experimental design

Establishing Baseline Operating Pressure (BOP)

The pressure in the balloon was increased from 4 mmHg in steps of 1 mmHg for 1 min per step until respiratory excursions were observed. The BOP was defined as 2 mmHg above the minimal distension pressure at which respiratory excursions were clearly recorded from the barostat tracing. If respiratory variations were not seen by 18 mmHg, BOP was set at 12 mmHg.

An initial “conditioning” distension of the colon was performed with pressure increased from 0 mmHg in steps of 4 mmHg for 15 s per step until 20 mmHg was reached. Previous studies have shown that this procedure renders subsequent assessments of compliance and perception more reproducible [16].

Ascending Method of Limits for Compliance and Sensory Thresholds

Colonic compliance and sensory thresholds were measured by stepwise inflation, starting at 0 mmHg and increasing in steps of 4 mmHg for 1 min per step to a maximum of 64 mmHg. Linear interpolation of the pressure volume curves were used to identify pressure at half maximum volume (Pr50 [17]). Thresholds for first sensation, gas, and pain were indicated by pressing a button when respective sensations were perceived.

Random Order Phasic Distensions: Sensory Ratings

Phasic distensions between 8 and 36 mmHg above BOP were applied once in random order provided by the study statistician (A.R.Z.), maintained for 60 s with an inter-stimulus interval of 2 min during which the balloon was deflated to BOP, a reliable method in which intensity ratings were generally proportional to the magnitude of the distension pressures [1820]. Subjects marked separate 100 mm visual analog scales (VAS) for the sensations of gas and pain 30 s after the onset of the distension. The ends of the scales were anchored by the terms “unnoticeable” and “unbearable”. Pressure was immediately released if the subject reported greater than 80 mm of pain on the VAS scale and higher distensions were not subsequently administered. During the assessment of sensation, the interaction between the subject and the study investigator was kept to a minimum.

Fasting Tone and Postprandial Change in Colonic Tone

Fasting tone for 30 min was measured as the baseline barostat balloon volume; measurement was obtained for two periods: during fasting at baseline and after ingestion of placebo (double-blinded treatment); and during 30 min after ingestion of a 1000-kcal liquid meal (milkshake). The change in barostat balloon baseline volume was an expression of the postprandial increase in tone.

Data Analysis

The following measurements were derived as the primary endpoints for analysis:

  1. 1

    sensory thresholds for first sensation, gas, and pain during ascending method of limits;

     
  2. 2

    sensation ratings for gas and pain in response to the random phasic distensions;

     
  3. 3

    colonic compliance; and

     
  4. 4

    fasting tone and postprandial change in colonic tone.

     

A summary value for compliance was computed by linear interpolation on the pressure volume curve [17] to identify the pressure at one half of the maximum observed volume (Pr50), where a smaller Pr50 corresponds to higher compliance.

Statistical Analysis

The association of gender with response was assessed using analysis of covariance models (compliance, tone, VAS sensation ratings) and proportional hazards regression models (sensation thresholds) including age and BMI as covariates in each model.

Coefficients of Variation

Inter-coefficient of variation (COV) was calculated as 100 (SD/mean), and intra-COV as (100 × SD delta/overall mean), where the overall mean is the mean of the per subject means of the pair of values used to compute the within subject deltas.

Results

Participant Demographics

A total of 38 males and 34 females were enrolled in the study and the participants were similar in regards to age, BMI, and racial/ethnic background (Table 1).
Table 1

Demographics of healthy volunteers included in the study

 

Overall

Males (n = 38)

Females (n = 34)

Race (% Caucasian)

86

87

85

Mean age (SD), year

33 (12)

33 (13)

33 (11)

Mean BMI (SD)

25.0 (4.0)

25.3 (3.4)

24.6 (4.6)

Colonic Motor and Sensory Functions and Effect of Gender

Data are summarized in Table 2. Note that the intra-COV was not available for PP tone and the change (fasting vs. PP), because there were only comparable post (placebo) data routinely obtained for the study of meal effects in each participant. Mean and standard deviation of baseline measurements are provided, except where noted; there were no statistically significant associations with gender for any of the baseline measurement of colonic compliance, tone or sensation thresholds or ratings except for fasting tone.
Table 2

Intra and Inter-individual coefficients of variation in colonic motor and sensory testing

Group

All participants, n = 72

Males, n = 38

Females, n = 34

Function

Mean ± SD

% COV inter/intra

Mean ± SD

% COV inter/intra

Mean ± SD

% COV inter/intra

Compliance Pr50, mmHg

19.7 ± 4.5

22.8/17.5

20.6 ± 4.9

23.7/17.0

18.8 ± 3.9

20.8/18.2

Fasting 30 min tonea, ml

113.4 ± 34.9

30.8/21.5

106 ± 31.9

30.2/19.5

122 ± 36.6

30.0/21.1

PP 30 min tonea, ml

74.5 ± 26.8

35.9/NA

70.7 ± 23.4

33.2/NA

78.8 ± 29.8

37.9/NA

Δ30 min PP tonea, ml

−38.9 ± 23.0

59.1/NA

−35.1 ± 20.9

59.6/NA

−43.1 ± 24.7

57.3/NA

1st threshold, mmHg

15.6 ± 14.4

91.9/90.2

17.1 ± 15.6

91.4/84.9

14.0 ± 12.9

92.0/98.1

Gas threshold, mmHg

24.0 ± 17.5

72.8/75.9

24.9 ± 18.5

74.1/72.7

22.9 ± 16.5

71.7/71.9

Pain threshold, mmHg

42.4 ± 13.2

31.0/30.6

43.6 ± 14.4

33.1/35.4

41.2 ± 11.7

28.4/27.2

Sensory ratings gas (mm VAS) at 36 mmHg distension

53.3 ± 22.3

41.9/36.6

53.8 ± 18.5

34.5/43.1

52.8. ± 25.9

49.1/29.1

Sensory ratings pain (mm VAS) at 36 mmHg distension

48.6 ± 27.3

56.3/33.0

49.4 ± 27.2

55.1/38.3

47.9 ± 28.1

58.6/27.1

aPost (placebo) data

COV coefficient of variation, SD standard deviation, VAS visual analog scale, NA not available

A modest association with gender was observed for baseline compliance (Pr50 values), P = 0.080, however this was not significant after adjusting for maximum pressure level attained in the AML sequence (P = 0.22). Significant associations of baseline compliance with age (P = 0.043) and BMI (P = 0.003) were observed even after adjusting for maximum pressure level. Similar results were observed for the post-placebo fasting data. No associations of gender with sensation VAS ratings or with sensation thresholds were detected, though age was associated with threshold levels.

A modest association of fasting tone levels and gender was observed (P = 0.040), but not with PP change in tone. No associations with age or BMI and colonic tone were observed.

COVs are generally lower for compliance, fasting tone, pain threshold, and sensation ratings than for PP tone and threshold for first or gas sensation. COV data are similar in males and females; sensation COVs appear smaller in females relative to males.

Sample Size Estimates for Future Studies

Based on the interindividual COVs, we have calculated in Table 3 the sample size required to demonstrate an effect size of 25–35% for the most relevant endpoints. For first sensation and gas threshold, the sample size to detect a 25% effect is greater than 100 per treatment group. Therefore, this information is excluded from the table.
Table 3

Number of subjects (per treatment group or per disease/phenotype) needed to detect listed effect sizea with ≥80% power (two-sided α = 0.05) based on a two-sample t-test

Response

CV inter (%)

Effect size (%) demonstrablea

N per group required

Compliance Pr50 mmHg

22.8

25

15

30

11

35

8

Fasting tone, ml

30.8

25

25

30

18

35

14

First 30 min postprandial change in tone vs. fasting, ml

59.1

25

89

30

62

35

46

Pain threshold, mmHg

31

25

26

30

18

35

14

Gas sensation rating with 36 mmHg distension

41.9

25

46

30

32

35

24

Pain sensation rating with 36 mmHg distension

56.3

25

81

30

57

35

42

Discussion

We observed substantial reproducibility in the testing of compliance, tone and pain, and gas sensation in left colon. The test performance is certainly adequate to assess these colonic functions in humans. The performance of sensation thresholds and postprandial responses are less reproducible, though the intra-individual COV is smaller than inter-individual COV, suggesting that crossover studies might be preferable for studies of sensation thresholds and postprandial tone. These data provide the basis for calculating sample sizes for future studies. Moreover, the lower COV for sensation tests among females is relevant to plan pharmacodynamic studies of drugs intended for patients with functional gastrointestinal disorders. Interestingly, there were no statistically significant gender differences in these sensory and motor endpoints.

The importance of this study is that it establishes the performance characteristics of different measurements of colonic motor and sensory functions in humans. These observations are relevant in research studies that evaluate the pharmacodynamic effects of novel medications. Moreover, measurements of colonic compliance and tone are also used in evaluating colonic motor function in adult patients, such as those with significant constipation, as reported in a recent consensus document on intraluminal motility measurements [21]. Studies of colonic motor function have also demonstrated clinical utility in pediatric gastroenterology practice. For example, colonic motility tests facilitate the selection of medical and surgical treatment when conventional medical and behavioral treatments have failed [22, 23]. Colonic motility tests may also be used to better understand the cause of persistent symptoms after removal of the aganglionic segment in children with Hirschsprung’s disease [24], to evaluate the function of the colon after diversion colostomy prior to closure of the stoma [25] and to predict the response to antegrade enemas via cecostomy [26].

Rectal sensation has been proposed as a biomarker for irritable bowel syndrome [11] and the performance of the studies has been tested in a two-center study conducted at University of Manchester, UK and the Mayo Clinic [12]. This paper provides the first documentation of the performance of sensory testing in the colon proximal to the rectum. While the study has a number of strengths including conduct by a single operator and the relatively large number of people evaluated, there are inherent weaknesses: first, results are not necessarily generalizable to other centers and second, they do not present inter-center variability data. The relevance of sensory testing to clinical practice may still be debatable since hypersensitivity is documented in a range of IBS patients from 21% to 94% in the published literature [2, 5, 8, 9, 27, 28]. However, if such sensation tests are to be used in diagnostic or research studies, it is clear that the performance, normal range, and coefficient of variation are key properties of interest.

There are other techniques that are proposed to measure rectosigmoid colonic sensation. A multimodal method was tested in 12 healthy volunteers (six men mean age 32 years) on two subsequent days [29]. Stimulation with electrical, thermal, and mechanical modalities resulted in different sensory perceptions; however, the relationship between stimulus intensity and sensory response was linear for all modalities, and sensory response to different modalities did not differ between investigation days. It is interesting to note that, for sensory threshold, the cross-sectional area showed the best reproducibility (intra-class correlation, ICC ≥ 0.9) and, at pain detection threshold, stretch ratio, CSA and electro stimulation showed best reproducibility (ICC = 1.0; 0.9; 0.9, respectively). On the other hand, cross-sectional area in the rectosigmoid maybe constrained by the effect of the uterus, if it is retroverted or has unsuspected myomas. Further studies in the colon, using this interesting multimodal approach are awaited.

One of the constraints of this study is that reproducibility was tested on the same day. While we cannot exclude the possibility that reproducibility may have differed if the tests were each conducted on separate days, the previous data for rectal sensitivity in a two-center study [12] and using multimodal rectal stimulation [29] suggests that the results on one day generally reflect the reproducibility observed when studies are repeated on different days.

Table 4 shows a review of papers in the literature (with far smaller sample sizes than documented in our study) that provide information on coefficient of variation for a few of the sensation endpoints specifically, thresholds for sensation in the papers published in the literature; none of the studies actually provide information on colonic compliance and postprandial tone or sensation ratings. Note that the COVs for first sensation and gas thresholds are generally larger, as observed in our study [3032]. In the study by Tack et al., two measurements were obtained at least two weeks apart [32], and the COVs appear to be much lower than in the study from Simren et al. and in this study.
Table 4

Literature review of studies showing coefficient of variation and effect sizes for estimating effects on threshold sensations

Thresholds

N

Mean

SD

COV, %

Effect size demonstrable with ≥80% powera

Ref.

First sensation

13

26.4

9.01

34.14

35%

Simren [30]

Gas

13

36.4

10.81

29.70

30%

 

Discomfort

13

43.3

8.29

19.15

20%

 

Pain

13

46.4

5.41

11.66

12%

 

Pain

34

36.61

10.61

28.99

19%

Grover [31]

First perception (Day 1)

10

7.1

3.48

48.99

56%

Tack [32]

First perception (Day 2—pre-infusion)

10

9.6

4.11

42.82

49%

 

aBased on a 2-sample t-test (2-sided alpha level of 0.05) assuming the listed sample size per group(N). Effect size is the difference in group means divided by the overall mean (in % scale)

The observations in our study apply to healthy subjects and may not be generalizable to IBS patients, in whom anxiety or fear of pain levels may be higher and in whom increased perception of non-noxious sensations has been reported [33]. It is also conceivable that variability may be significantly greater in IBS patients due to a variety of factors unrelated to the stimulus.

In conclusion, we have reported the largest set of reproducibility data on colonic sensation and motility in humans to date, and we find that the results in healthy volunteers are generally reproducible, particularly for measurements of fasting tone, compliance and sensation ratings and pain threshold. Though reproducibility of other thresholds and postprandial tone response is not as good, assessment of variation allows for the use of these endpoints in adequately sized studies of experimental therapies or in studies comparing health and diseases such as irritable bowel syndrome. The lower intra-COV for sensations in females supports the use of these endpoints and methods in pharmacodynamic proof-of-concept studies or in dose-response studies in healthy volunteers. The lower COV for sensation tests among females is relevant to plan studies of drugs intended for functional GI disorders. Such studies may be useful prior to pursuing similar pharmacodynamic studies or clinical trials in patients with functional gastrointestinal disorders. The availability of such biomarkers is identified as an item of priority interest for the development of novel treatments in patients with lower functional gastrointestinal disorders, like irritable bowel syndrome [34].

Acknowledgments

Dr Camilleri’s work on colon function is supported by grants R01-DK67071 and K24-DK02638 from National Institutes of Health. Dr Papathanasopoulos is supported by an international grant of the Hellenic Society of Gastroenterology

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© US Government 2009