Digestive Diseases and Sciences

, Volume 54, Issue 10, pp 2198–2206

Down-regulation of Protein and mRNA Expression of IL-8 and ICAM-1 in Colon Tissue of Ulcerative Colitis Patients by Partition-Herb Moxibustion

  • En-Hua Zhou
  • Hui-Rong Liu
  • Huan-Gan Wu
  • Zheng Shi
  • Wei Zhang
  • Yi Zhu
  • Da-Ren Shi
  • Shuang Zhou
Original Article

DOI: 10.1007/s10620-008-0620-4

Cite this article as:
Zhou, EH., Liu, HR., Wu, HG. et al. Dig Dis Sci (2009) 54: 2198. doi:10.1007/s10620-008-0620-4

Abstract

Our previous studies have demonstrated the efficacy of partition-herb moxibustion on ulcerative colitis (UC) rats. However, the mechanism of actions of partition-herb moxibustion is still unclear. Most Chinese acupuncturists believe that partition-herb improves the effect of moxibustion therapy. However, this argument lacks confirming experimental and clinical evidence. So, whether partition-herb does play a role in the mechanism of actions of partition-herb moxibustion was reported in this paper. A total of 123 patients were randomly divided into the partition-herb moxibustion group and the control group (partition-bran moxibustion group). Fourteen patients dropped out of the study. Finally, 109 patients finished the intervention. Sixty-one patients with UC received partition-herb moxibustion and 48 patients with UC received partition-bran moxibustion for 72 treatments, one treatment per day, every 12 treatments with an interval of 3 days. The expressions of interleukin-8 (IL-8) and intercellular adhesion molecule-1 (ICAM-1) in the colonic mucosa tissue in mild patients with UC were assayed by immunohistochemistry and RT-PCR. Histology of the colon also was observed at entry and after 72 treatments. Clinical therapeutic effect in partition-herb moxibustion was significantly better than those in partition-bran moxibustion (< 0.05). Protein and mRNA expression of IL-8, ICAM-1 in the patients was inhibited by both partition-herb moxibustion and partition-bran moxibustion, of those the inhibition in the partition-herb moxibustion group was more obvious (< 0.01). Partition-herb moxibustion was shown to significantly improve the histology of the colon, and partition-herb was shown to improve the therapeutic effect of moxibustion on treatment of UC patients.

Keywords

Ulcerative colitisMoxibustionPartition-herb moxibustionGene expressionImmunohistochemistryRT-PCR

Abbreviations

ICAM-1

Intercellular adhesion molecule-1

IL-8

Interleukin-8

UC

Ulcerative colitis

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • En-Hua Zhou
    • 1
  • Hui-Rong Liu
    • 1
  • Huan-Gan Wu
    • 1
  • Zheng Shi
    • 1
  • Wei Zhang
    • 2
  • Yi Zhu
    • 1
  • Da-Ren Shi
    • 3
  • Shuang Zhou
    • 1
  1. 1.Shanghai Institute of Acupuncture-Moxibustion and MeridiansShanghaiPeople’s Republic of China
  2. 2.Acupuncture DepartmentXiamen Hosptial of T.C.MXiamenPeople’s Republic of China
  3. 3.Cancer HospitalFudan UniversityShanghaiPeople’s Republic of China