Digestive Diseases and Sciences

, Volume 54, Issue 2, pp 255–263

The Effect of Saccharomyces boulardii on Human Colon Cells and Inflammation in Rats with Trinitrobenzene Sulfonic Acid-Induced Colitis

Original Article

DOI: 10.1007/s10620-008-0357-0

Cite this article as:
Lee, S.K., Kim, Y.W., Chi, S. et al. Dig Dis Sci (2009) 54: 255. doi:10.1007/s10620-008-0357-0

Abstract

Saccharomyces boulardii (S. boulardii) has beneficial effects in the treatment of intestinal inflammation; however, little is known about the mechanisms by which these effects occur. We investigated the effects of S. boulardii on the expression of peroxisome proliferator-activated receptor-gamma (PPAR-γ) and interleukin-8 (IL-8), using human HT-29 colonocytes and a rat model of trinitrobenzene sulfonic acid (TNBS)-induced colitis. The effect of S. boulardii on gene expression was assessed by semi-quantitative reverse transcription–polymerase chain reaction (RT-PCR), and Northern blot and Western blot assays. Pharmacological inhibitors for various signaling pathways were used to determine the signaling pathways implicated in the S. boulardii regulation of PPAR-γ and IL-8. We found that S. boulardii up-regulated and down-regulated PPAR-γ and IL-8 expression at the transcription level, both in vitro and in vivo (P < 0.05, respectively). Saccharomyces boulardii blocked tumor necrosis factor-alpha (TNF-α) regulation of PPAR-γ and IL-8 through disruption of TNF-α-mediated nuclear factor kappa B (NF-κB) activation. Furthermore, S. boulardii suppressed colitis and expression of pro-inflammatory cytokine genes in vivo (P < 0.05, respectively). Our study demonstrated that S. boulardii reduces colonic inflammation and regulates inflammatory gene expression.

Keywords

Saccharomyces boulardiiPPAR-γIL-8HT-29TNBS-induced colitis

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Department of Internal Medicine, Institute of GastroenterologyYonsei University College of MedicineSeoulKorea
  2. 2.Department of Pathology, School of MedicineKyung Hee UniversitySeoulKorea
  3. 3.School of Life Sciences and BiotechnologyKorea UniversitySeoulKorea
  4. 4.Department of Internal Medicine, School of MedicineKyung Hee UniversitySeoulKorea