Original Paper

Digestive Diseases and Sciences

, Volume 53, Issue 7, pp 1801-1810

First online:

Akt1/protein Kinase Bα is Involved in Gastric Cancer Progression and Cell Proliferation

  • Zheyi HanAffiliated withThe State Key Laboratory for Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University
  • , Kaichun WuAffiliated withThe State Key Laboratory for Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University Email author 
  • , Huiqin ShenAffiliated withThe State Key Laboratory for Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University
  • , Chunying LiAffiliated withDepartment of Dermatology, Xijing Hospital, Fourth Military Medical University
  • , Shuang HanAffiliated withThe State Key Laboratory for Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University
  • , Liu HongAffiliated withThe State Key Laboratory for Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University
  • , Yongquan ShiAffiliated withThe State Key Laboratory for Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University
  • , Na LiuAffiliated withThe State Key Laboratory for Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University
  • , Changcun GuoAffiliated withThe State Key Laboratory for Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University
    • , Yan XueAffiliated withThe State Key Laboratory for Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University
    • , Taidong QiaoAffiliated withThe State Key Laboratory for Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University
    • , Daiming FanAffiliated withThe State Key Laboratory for Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University Email author 

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Abstract

Akt (also known as protein kinase B, PKB) is involved in a variety of biological processes, for example cell development, proliferation, and angiogenesis. Clinical studies in support of the idea that increased activity of Akt could contribute directly to gastric carcinogenesis are rare, however. In this study we discovered that phospho-Akt1 was overexpressed in human gastric cancers and its levels correlated with tumor differentiation and pTNM. Akt1 activation promoted cell survival, because the phosphatidylinositol 3-kinase(PI3K) inhibitor LY294002 inhibited Akt1 phosphorylation and inhibited cell growth, especially in cells with active Akt1. Dominant negative Akt inhibited proliferation of gastric cancer cells and induced G1 cell-cycle arrest whereas constitutively active Akt increased cell proliferation. We have therefore identified Akt1 as an active kinase that contributes to gastric cancer progression and promotes proliferation of gastric cancer cells.

Keywords

Akt1 Protein kinase Bα Gastric cancer Proliferation Cell cycle