Digestive Diseases and Sciences

, Volume 52, Issue 5, pp 1242–1246

Activation of β-Catenin in the Stem Cell Region of Crypts During Growth of the Small Intestine in Infant Rats

Authors

  • Kimberley S. Camac
    • Department of Gastroenterology and HepatologyThe Queen Elizabeth Hospital
    • University of South Australia
  • Fiona M. Thompson
    • Department of Gastroenterology and HepatologyThe Queen Elizabeth Hospital
    • University of Adelaide
    • Department of Gastroenterology and HepatologyThe Queen Elizabeth Hospital
    • University of South Australia
    • University of Adelaide
    • Department of Gastroenterology and HepatologyThe Queen Elizabeth Hospital
Original Paper

DOI: 10.1007/s10620-006-9200-7

Cite this article as:
Camac, K.S., Thompson, F.M. & Cummins, A.G. Dig Dis Sci (2007) 52: 1242. doi:10.1007/s10620-006-9200-7

Abstract

The Wnt/β-catenin pathway has been proposed as promoting intestinal stem cell division. Wnt ligands activate cytoplasmic β-catenin and increase nuclear translocation of β-catenin that binds to the Tcf-4 transcription factor. The aim of this study was to investigate β-catenin expression in the stem cell region of crypts during intestinal growth in rats. Litters of DAxPVG/c rats were humanely killed at 7, 14, 21, 35, and 72 days of life. β-Catenin and Tcf-4 were quantified by immunoperoxidase staining and image analysis with cumulative signal analysis. Cytoplasmic and nuclear expression of β-catenin peaked nearly 2-fold at day 14 (versus day 7) of life in the stem cell region of intestinal crypts. Tcf-4 nuclear expression peaked earlier at 7 days and was lower thereafter with age. We conclude that the Wnt/β-catenin pathway is activated in the stem cell region of intestinal crypts during growth of the small intestine.

Keywords

Beta cateninGrowth factorIntestinal growthIntestinal stem cell

Copyright information

© Springer Science+Business Media, Inc. 2006