Inflammatory Bowel Disease

Digestive Diseases and Sciences

, Volume 51, Issue 4, pp 677-686

An Inverse Correlation of Human Peripheral Blood Regulatory T Cell Frequency with the Disease Activity of Ulcerative Colitis

  • Makoto TakahashiAffiliated withDepartment of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University
  • , Kazuhiko NakamuraAffiliated withDepartment of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu UniversityDepartment of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University Email author 
  • , Kuniomi HondaAffiliated withDepartment of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University
  • , Yousuke KitamuraAffiliated withDepartment of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University
  • , Takahiro MizutaniAffiliated withDepartment of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University
  • , Yuzuru ArakiAffiliated withNational Fukuoka-Higashi Medical Center
  • , Teppei KabemuraAffiliated withSaiseikai Fukuoka General Hospital
  • , Yoshiharu ChijiiwaAffiliated withHara Sanshin Hospital
  • , Naohiko HaradaAffiliated withDepartment of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu UniversityNational Fukuoka-Higashi Medical Center
    • , Hajime NawataAffiliated withDepartment of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University

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Abstract

Evidence suggests that CD4+CD25+ regulatory T cells play a crucial role in the suppression of intestinal inflammation. However, their role in the suppression of inflammatory bowel disease has not yet been addressed. We examined the proportion of regulatory T cells in inflammatory bowel disease. First, we isolated CD4+CD45RO+CD25+ T cells from the peripheral blood of healthy persons and showed that these cells suppressed T cell proliferation profoundly and expressed FoxP3 abundantly, revealing that they are regulatory cells. Then the proportion of CD45RO+CD25+ in peripheral blood CD4+ T cells was analyzed in patients and healthy controls by flow cytometry. CD4+CD45RO+CD25+ T cell frequency was significantly lower in active ulcerative colitis than in the control and inactive ulcerative colitis. CD4+CD45RO+CD25+ T cell frequency was inversely correlated with the clinical and endoscopic severity of ulcerative colitis. These results suggest that a deficiency of regulatory T cells is associated with the progression of ulcerative colitis.

Key Words

inflammatory bowel disease colitis ulcerative CD4-positive T lymphocyte interleukin-2 receptor α