Digestive Diseases and Sciences

, Volume 50, Issue 6, pp 1113–1117

Evaluation of Five Probiotic Products for Label Claims by DNA Extraction and Polymerase Chain Reaction Analysis

Authors

    • University of Kansas Medical Center
    • Program in Integrative MedicineUniversity of Knasas Medical Center
  • Bette Bischoff
    • University of Kansas Medical Center
  • Cheryl Giles
    • University of Kansas Medical Center
  • Martin Adelson
    • Medical Diagnostic Laboratories
  • Raja-Venkitesh S. Rao
    • Medical Diagnostic Laboratories
  • Richard Mccallum
    • University of Kansas Medical Center
Article

DOI: 10.1007/s10620-005-2714-6

Cite this article as:
Drisko, J., Bischoff, B., Giles, C. et al. Dig Dis Sci (2005) 50: 1113. doi:10.1007/s10620-005-2714-6
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Abstract

Label claims were evaluated for five probiotic products. Specific oligonucleotide primers were designed for 11 species from the Bifidobacterium, Lactobacillus, and Streptococcus genera. Polymerase chain reaction, gel electrophoresis, and amplicon excision with DNA sequencing were performed: Sequence analysis and DNA homology comparisons followed. Bifidobacterium bifidum was not detected in two of the five samples by PCR analysis. Also, Lactobacillus species were found in two of the five product samples for which the species was not listed as an ingredient. We conclude that (1) lack of B. bifidum in two probiotic products may be attributed to different preparation standards among probiotic manufacturers, and (2) identificaition of additional Lactobacillus species may represent contamination of the samples related to the fact that manufacturers utilize shared equipment to produce all probiotics and PCR is a highly sensitive technique.

Key Words

DNA PCRprobioticsupplement label claimsLactobacillusBifidobacterium species

Copyright information

© Springer Science + Business Media, Inc. 2005