Cytotechnology

, Volume 65, Issue 4, pp 609–619

Role of phytoestrogenic oils in alleviating osteoporosis associated with ovariectomy in rats

  • Hanaa A. Hassan
  • Azza M. EL Wakf
  • Nermin E. EL Gharib
Original Research

DOI: 10.1007/s10616-012-9514-6

Cite this article as:
Hassan, H.A., EL Wakf, A.M. & EL Gharib, N.E. Cytotechnology (2013) 65: 609. doi:10.1007/s10616-012-9514-6

Abstract

The objective of this study was to elucidate the effect of soybean oil (SbO) and sesame oil (SO) supplemented diets on bone biomarkers changes in OVX (ovariectomized) rats. The current data exhibited significant decrease in BMD (bone mineral density), accompanied with marked depletion in the level of Ca, P and Mg in both serum and bone of OVX rats. Also, serum estrogen, total protein, HDL-C (high density lipoprotein cholesterol), bone NO levels were decreased in OVX rats. However, a significant increase in the level of serum TL (total lipids), TC (total cholesterol), TG (triglycerides), LDL-C (low density lipoprotein cholesterol), VLDL-C (very low density lipoprotein cholesterol), urine minerals (Ca, P, Mg), as well as serum, bone and urine ALP (alkaline phosphatase) and ACP (acid phosphatase) activity were recorded in OVX rats. Further changes were also detected by the increased level of urine hydroxyproline, serum parathyroid hormone and osteocalcin, as well as urea and creatinine level in both serum and urine. On the other hand, when OVX rats were fed on SbO (soy bean oil) (15 % w/w) or SO (sesame oil) (10 % w/w) supplemented diets, the data recorded a significant improvement in all the above mentioned parameters. So, it can be concluded that consumption of SbO or SO supplemented diets might be considered as a functional food for retarding risks of osteoporosis associated with estrogen deficiency in OVX states.

Keywords

Ovariectomyized ratEstrogen deficiencySoybean oilSesame oil osteoporosis

Abbreviations

NC

Normal control

SbO

Soy bean oil

SO

Sesame oil

OVX

Ovariectomized

BMD

Bone mineral density

TL

Total lipids

TC

Total cholesterol

TG

Triglycerides

HDL-C

High density lipoprotein cholesterol

LDL-C

Low density lipoprotein cholesterol

VLDL-C

Very low density lipoprotein cholesterol

ALP

Alkaline phosphatase

ACP

Acid phosphatase

NO

Nitric oxide

Ca

Calcium

P

Phosphorus

Mg

Magnesium

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Hanaa A. Hassan
    • 1
  • Azza M. EL Wakf
    • 1
  • Nermin E. EL Gharib
    • 1
  1. 1.Physiology Division, Zoology Department, Faculty of ScienceMansoura UniversityMansouraEgypt